ARG56590

anti-CCL2 / MCP1 antibody [2.2-4H5-1A11]

anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Cow,Human,Mouse

publication_link 参考文献2

概述

产品描述 Mouse Monoclonal antibody [2.2-4H5-1A11] recognizes CCL2 / MCP1
反应物种 Hu, Ms, Cow
应用 ELISA, IHC-P, WB
宿主 Mouse
克隆 Monoclonal
克隆号 2.2-4H5-1A11
同位型 IgG1, kappa
靶点名称 CCL2 / MCP1
抗原物种 Human
抗原 E.coli derived Recombinant Human CCL2 / MCP1.
(QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT)
偶联标记 Un-conjugated
別名 MCP1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; Monocyte secretory protein JE; HSMCR30; Small-inducible cytokine A2; HC11; SMC-CF; GDCF-2; SCYA2; C-C motif chemokine 2; Monocyte chemoattractant protein 1; MCP-1

应用说明

应用建议
应用 推荐稀释比
ELISASandwich: 2.0 - 4.0 µg/ml with ARG56731 as a detection antibody
IHC-P10.0 µg/ml
WB0.20 - 0.40 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 20296 Mouse CCL2

GeneID: 6347 Human CCL2

Swiss-port # P10148 Mouse C-C motif chemokine 2

Swiss-port # P13500 Human C-C motif chemokine 2

基因名称 CCL2
全名 chemokine (C-C motif) ligand 2
背景介绍 This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
生物功能 Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. [UniProt]
产品亮点 Related products:
MCP1 antibodies; MCP1 ELISA Kits; MCP1 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
预测分子量 11 kDa (Human); 16 kDa (Mouse)
翻译后修饰 Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

检测图片 (6) Click the Picture to Zoom In

  • ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] concentration is 10.0 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] WB image

    Western blot: 250 - 0.24 ng (left to right) of Human MCP-1 stained with ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11], under reducing conditions.

  • ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] concentration is 10.0 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] concentration is 10.0 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] WB image

    Western blot: 250 - 0.24 ng (left to right) of Human MCP-1 stained with ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11], under non-reducing conditions.

  • ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] standard curve image

    Sandwich ELISA: ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] as a capture antibody at 2.0 - 4.0 µg/ml combined with ARG56731 anti-CCL2 / MCP1 antibody (Biotin) as a detection antibody at ~ 0.5 - 1.0 µg/ml. Results of a typical standard run with optical density.

文献引用

Exosomal miR-7002-5p derived from highglucose-induced macrophages suppresses autophagy in tubular epithelial cells by targeting Atg9b

IHC-P / Mouse

Jing Zhao  et al.
FASEB J.,  (2022)

publication_link

 

hr_line

Ramulus Mori (Sangzhi) Alkaloids (SZ-A) Ameliorate Glucose Metabolism Accompanied by the Modulation of Gut Microbiota and Ileal Inflammatory Damage in Type 2 Diabetic KKAy Mice.

WB / Mouse

Quan Liu et al.
Front Pharmacol.,  (2021)

publication_link

 

hr_line