概述

产品描述	Rabbit Polyclonal antibody recognizes CBLB
反应物种	Hu, Ms, Rat
应用	WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	CBLB
抗原物种	Human
抗原	Recombinant Protein of Human CBLB.
偶联标记	Un-conjugated
別名	Nbla00127; EC 6.3.2.-; RNF56; Signal transduction protein CBL-B; Casitas B-lineage lymphoma proto-oncogene b; SH3-binding protein CBL-B; Cbl-b; E3 ubiquitin-protein ligase CBL-B; RING finger protein 56

应用说明

应用建议	应用 推荐稀释比 WB 1:500 - 1:2000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照	Jurkat

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 171136 Rat CBLB GeneID: 208650 Mouse CBLB GeneID: 868 Human CBLB Swiss-port # Q13191 Human E3 ubiquitin-protein ligase CBL-B Swiss-port # Q3TTA7 Mouse E3 ubiquitin-protein ligase CBL-B Swiss-port # Q8K4S7 Rat E3 ubiquitin-protein ligase CBL-B More
基因名称	CBLB
全名	Cbl proto-oncogene B, E3 ubiquitin protein ligase
生物功能	E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. [UniProt]
预测分子量	109 kDa
翻译后修饰	Phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation. Auto-ubiquitinated upon EGF-mediated cell activation or upon T-cell costimulation by CD28; which promotes proteasomal degradation.

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