ARG57312

anti-CBLB antibody

anti-CBLB antibody for Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes CBLB
反应物种 Hu, Ms, Rat
应用 WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 CBLB
抗原物种 Human
抗原 Recombinant Protein of Human CBLB.
偶联标记 Un-conjugated
別名 Nbla00127; EC 6.3.2.-; RNF56; Signal transduction protein CBL-B; Casitas B-lineage lymphoma proto-oncogene b; SH3-binding protein CBL-B; Cbl-b; E3 ubiquitin-protein ligase CBL-B; RING finger protein 56

应用说明

应用建议
应用 推荐稀释比
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Jurkat

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 171136 Rat CBLB

GeneID: 208650 Mouse CBLB

GeneID: 868 Human CBLB

基因名称 CBLB
全名 Cbl proto-oncogene B, E3 ubiquitin protein ligase
生物功能 E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. [UniProt]
预测分子量 109 kDa
翻译后修饰 Phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.
Auto-ubiquitinated upon EGF-mediated cell activation or upon T-cell costimulation by CD28; which promotes proteasomal degradation.

检测图片 (1) Click the Picture to Zoom In

  • ARG57312 anti-CBLB antibody WB image

    Western blot: Jurkat cell lysate stained with ARG57312 anti-CBLB antibody.