ARG53996
anti-CARM1 / PRMT4 antibody
anti-CARM1 / PRMT4 antibody for Immunoprecipitation,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
参考文献2
概述
| 产品描述 | Mouse Monoclonal antibody recognizes CARM1 / PRMT4 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1 |
| 靶点名称 | CARM1 / PRMT4 |
| 抗原物种 | Human |
| 抗原 | E.coli derived recombinant human PRMT4/CARM1 protein fragments. |
| 偶联标记 | Un-conjugated |
| 別名 | Protein arginine N-methyltransferase 4; EC 2.1.1.125; PRMT4; EC 2.1.1.-; Coactivator-associated arginine methyltransferase 1; Histone-arginine methyltransferase CARM1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 100 mM Tris (pH 7.4), 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol and 0.2% Sodium azide. |
| 抗菌剂 | 0.2% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q86X55 Human Histone-arginine methyltransferase CARM1 Swiss-port # Q9WVG6 Mouse Histone-arginine methyltransferase CARM1 |
|---|---|
| 基因名称 | CARM1 |
| 全名 | coactivator-associated arginine methyltransferase 1 |
| 背景介绍 | Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs |
| 生物功能 | Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm |
| 产品亮点 | Related products: PRMT4 antibodies; Anti-Mouse IgG secondary antibodies; Related news: TCA intermediate fumarate promotes mitobiogenesis |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 66 kDa |
| 翻译后修饰 | Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing (By similarity). Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol. |
检测图片 (1) Click the Picture to Zoom In
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ARG53996 anti-CARM1 / PRMT4 antibody WB image
Western blot: PANC-1/shControl and PANC-1/shCARM1 cell lysate stained with ARG53996 anti-CARM1 / PRMT4 antibody. Data showed CARM1 signal was decreased in CARM1 knockdown cells (PANC-1/shCARM1).
Data from: Wang YP et al. Arginine Methylation of MDH1 by CARM1 Inhibits Glutamine Metabolism and Suppresses Pancreatic Cancer. Molecular Cell 2016, 64 , 673–687.
文献引用
Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.
WB / Mouse
Arginine Methylation of MDH1 by CARM1 Inhibits Glutamine Metabolism and Suppresses Pancreatic Cancer.
WB / Human
