ARG58350
anti-CAD antibody
anti-CAD antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CAD |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CAD |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1650-1900 of Human CAD (NP_004332.2). |
| 偶联标记 | Un-conjugated |
| 別名 | EC 2.1.3.2; EC 6.3.5.5; CAD protein [Includes: Glutamine-dependent carbamoyl-phosphate synthase; EC 3.5.2.3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | SW620 | ||||||||
| 实际分子量 | 243 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CAD |
| 全名 | carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase |
| 背景介绍 | The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] |
| 生物功能 | This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase). [UniProt] |
| 细胞定位 | Cytoplasm, Nucleus. [UniProt] |
| 预测分子量 | 243 kDa |
| 翻译后修饰 | Activated by MAP kinase (Erk1/2) phosphorylation just prior to the S phase of the cell cycle, when the demand for pyrimidine nucleotides is greatest, and down-regulated as the cells emerge from S phase by protein kinase A (PKA) phosphorylation. Phosphorylation at Ser-1859 by RPS6KB1 downstream of MTOR promotes oligomerization and stimulates dihydroorotase activity. Phosphorylation at Ser-1406 reduces sensitivy to feedback inhibition by UTP. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG58350 anti-CAD antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver stained with ARG58350 anti-CAD antibody at 1:100 dilution.
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ARG58350 anti-CAD antibody WB image
Western blot: 25 µg of SW620 cell lysate stained with ARG58350 anti-CAD antibody at 1:1000 dilution.
