ARG45229

anti-C1orf77 / FOP antibody

anti-C1orf77 / FOP antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey

概述

产品描述 Rabbit Polyclonal antibody recognizes C1orf77 / FOP
反应物种 Hu, Ms, Rat, Mk
应用 FACS, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 C1orf77 / FOP
抗原物种 Human
抗原 Synthetic peptide corresponding to N-terminal region of human C1orf77 / FOP .
偶联标记 Un-conjugated
別名 CHTOP; Chromatin Target Of PRMT1; SRAG; FOP; C1orf77; Small Protein Rich In Arginine And Glycine; Chromatin Target Of PRMT1 Protein; Friend Of PRMT1 Protein; DKFZP547E1010; Small Arginine- And Glycine-Rich Protein; Chromosome 1 Open Reading Frame 77; Friend Of Prmt1; C10orf77; FL-SRAG; SRAG-3; SRAG-5; Pp7704

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 28 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 26097 Human CHTOP

GeneID: 66511 Mouse CHTOP

Swiss-port # Q9CY57 Mouse Chromatin target of PRMT1 protein

Swiss-port # Q9Y3Y2 Human Chromatin target of PRMT1 protein

基因名称 CHTOP
全名 Chromatin Target Of PRMT1
背景介绍 This gene encodes a small nuclear protein that is characterized by an arginine and glycine rich region. This protein may have an important role in the regulation of fetal globin gene expression and in the activation of estrogen-responsive genes. A recent study reported that this protein binds 5-hydroxymethylcytosine (5hmC) and associates with an arginine methyltransferase complex (methylosome), which promotes methylation of arginine 3 of histone H4 (H4R3) and activation of genes involved in glioblastomagenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
生物功能 Required for effective mRNA nuclear export and is a component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Stimulates DDX39B ATPase and helicase activities. In cooperation with ALYREF/THOC4 enhances NXF2 RNA binding activity. [Uniprot]
细胞定位 Nucleus. [Uniprot]
预测分子量 26 kDa
翻译后修饰 Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation. [Uniprot]

检测图片 (7) Click the Picture to Zoom In

  • ARG45229 anti-C1orf77 / FOP antibody IHC-P image

    Immunohistochemistry: Human breast cancer stained with ARG45229 anti-C1orf77 / FOP antibody at 2 μg/ml dilution.

  • ARG45229 anti-C1orf77 / FOP antibody WB image

    Western blot: Hela, THP-1, and MOLT-4 stained with ARG45229 anti-C1orf77 / FOP antibody at 0.5 μg/ml dilution.

  • ARG45229 anti-C1orf77 / FOP antibody FACS image

    Flow Cytometry: THP-1 stained with ARG45229 anti-C1orf77 / FOP antibody at 1 µg/10^6 cells dilution.

  • ARG45229 anti-C1orf77 / FOP antibody WB image

    Western blot: Rat brain and PC-12 stained with ARG45229 anti-C1orf77 / FOP antibody at 0.5 μg/ml dilution.

  • ARG45229 anti-C1orf77 / FOP antibody FACS image

    Flow Cytometry: RH35 stained with ARG45229 anti-C1orf77 / FOP antibody at 1 µg/10^6 cells dilution.

  • ARG45229 anti-C1orf77 / FOP antibody WB image

    Western blot: Mouse spleen and mouse brain stained with ARG45229 anti-C1orf77 / FOP antibody at 0.5 μg/ml dilution.

  • ARG45229 anti-C1orf77 / FOP antibody WB image

    Western blot: COS-7 stained with ARG45229 anti-C1orf77 / FOP antibody at 0.5 μg/ml dilution.