ARG59534
anti-Brachyury antibody
anti-Brachyury antibody for ICC/IF,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Brachyury |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Brachyury |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 15-43 of Human Brachyury. |
| 偶联标记 | Un-conjugated |
| 別名 | Protein T; TFT; Brachyury protein; SAVA |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | NCI-H460 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | T |
| 全名 | T brachyury transcription factor |
| 背景介绍 | The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012] |
| 生物功能 | Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 47 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG59534 anti-Brachyury antibody ICC/IF image
Immunofluorescence: HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG59534 anti-Brachyury antibody (green) at 1:25, 1 hour at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 hour at 37°C). Nuclei were counterstained with DAPI (blue).
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ARG59534 anti-Brachyury antibody WB image
Western blot: 20 µg of NCI-H460 cell lysate stained with ARG59534 anti-Brachyury antibody at 1:2000 dilution.
