ARG45539

anti-Borealin / CDCA8 antibody

anti-Borealin / CDCA8 antibody for Flow cytometry,ICC/IF,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes Borealin / CDCA8
反应物种 Hu
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Borealin / CDCA8
抗原物种 Human
抗原 Recombinant protein containing to human Borealin / CDCA8.
偶联标记 Un-conjugated
別名 CDCA8; Cell Division Cycle Associated 8; Borealin; MESRGP; DasraB; BOR; Pluripotent Embryonic Stem Cell-Related Gene 3 Protein; Cell Division Cycle-Associated Protein 8; FLJ12042; HDasra-B; Dasra-B; Dasra B; PESCRG3

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 35 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 55143 Human CDCA8

Swiss-port # Q53HL2 Human Borealin

基因名称 CDCA8
全名 Cell Division Cycle Associated 8
背景介绍 This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. [provided by RefSeq, Apr 2013]
生物功能 Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. [UniProt]
细胞定位 Centromere; Chromosome; Cytoplasm; Cytoskeleton; Nucleus. [UniProt]
预测分子量 31 kDa
翻译后修饰 Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG45539 anti-Borealin / CDCA8 antibody ICC/IF image

    Immunofluorescence: U2OS stained with ARG45539 anti-Borealin / CDCA8 antibody at 5 μg/ml dilution.

  • ARG45539 anti-Borealin / CDCA8 antibody WB image

    Western blot: K562 stained with ARG45539 anti-Borealin / CDCA8 antibody at 0.5 μg/ml dilution.

  • ARG45539 anti-Borealin / CDCA8 antibody FACS image

    Flow Cytometry: K562 stained with ARG45539 anti-Borealin / CDCA8 antibody at 1 µg/10^6 cells dilution.