ARG58309
anti-Bmi 1 antibody
anti-Bmi 1 antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Bmi 1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Bov |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Bmi 1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence in the middle region of Human Bmi1(135-165aa IEFFDQNRLDRKVNKDKEKSKEEVNDKRYLR), different from the related Mouse sequence by four amino acids. |
| 偶联标记 | Un-conjugated |
| 別名 | PCGF4; RNF51; FLVI2/BMI1; flvi-2/bmi-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BMI1 |
| 全名 | BMI1 proto-oncogene, polycomb ring finger |
| 背景介绍 | This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015] |
| 生物功能 | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:15386022, PubMed:16359901, PubMed:26151332, PubMed:16714294, PubMed:21772249, PubMed:25355358, PubMed:27827373). The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed:21772249, PubMed:25355358). In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed:15386022, PubMed:26151332, PubMed:21772249). [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. [UniProt] |
| 预测分子量 | 37 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG58309 anti-Bmi 1 antibody WB image
Western blot: 0.5 ng of Recombinant Human Bmi1 protein stained with ARG58309 anti-Bmi 1 antibody at 0.5 µg/ml dilution.
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ARG58309 anti-Bmi 1 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat brain and Mouse brain lysates stained with ARG58309 anti-Bmi 1 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG58309 anti-Bmi 1 antibody WB image
Western blot: 50 µg of Rat spleen, 40 µg of HT1080 whole cell lysate and 40 µg of HeLa whole cell lysate stained with ARG58309 anti-Bmi 1 antibody at 0.5 µg/ml dilution.
