ARG54434
anti-Bmf antibody
anti-Bmf antibody for ICC/IF,Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Bmf |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 特异性 | This antibody recognizes an epitope at the C-terminus of human Bmf (Bcl-2-Modifying Factor), a novel BH3-only protein. In healthy cells, Bmf associates with the dynein light chain 2 component of the myosin V motors and is sequestered by the cell’s actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiaing the downstream apoptotic program. Bmf is constitutively expressed in many tissues. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Bmf |
| 抗原物种 | Human |
| 抗原 | Peptide corresponding to aa 171-184 of human Bmf (accession no. NP_277038). |
| 偶联标记 | Un-conjugated |
| 別名 | Bcl-2-modifying factor |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HepG2 and Human kidney |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunoaffinity chroma-tography |
| 缓冲液 | PBS (pH 7.4) and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BMF |
| 全名 | Bcl2 modifying factor |
| 背景介绍 | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a single BCL2 homology domain 3 (BH3), and has been shown to bind BCL2 proteins and function as an apoptotic activator. This protein is found to be sequestered to myosin V motors by its association with dynein light chain 2, which may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| 生物功能 | May play a role in apoptosis. Isoform 1 seems to be the main initiator. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody |
| 预测分子量 | 21 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG54434 anti-Bmf antibody WB image
Western Blot: HepG2 cell lysate stained with ARG54434 anti-Bmf antibody at (A) 2.5 and (B) 5 μg/ml dilution.
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ARG54434 anti-Bmf antibody IHC image
Immunohistochemistry: human kidney stained with ARG54434 anti-Bmf antibody at 10 μg/ml dilution.
