ARG63743
anti-Bid antibody
anti-Bid antibody for ICC/IF,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Bid |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 特异性 | This antibody is expected to recognise all three reported isoforms (as represented by NP_932070.1; NP_001187.1; NP_932071.1) |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Bid |
| 抗原物种 | Human |
| 抗原 | C-TYVRSLARNGMD |
| 偶联标记 | Un-conjugated |
| 別名 | p22 BID; BID; p15 BID; p11 BID; BH3-interacting domain death agonist; p13 BID; FP497 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P55957 Human BH3-interacting domain death agonist |
|---|---|
| 背景介绍 | This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody |
| 预测分子量 | 22 kDa |
| 翻译后修饰 | TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products. p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation. |
检测图片 (2) Click the Picture to Zoom In
-
ARG63743 anti-Bid antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton. Cells were stained with ARG63743 anti-Bid antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
-
ARG63743 anti-Bid antibody WB image
Western blot: 35 µg of A431 cell lysate (protein in RIPA buffer) stained with ARG63743 anti-Bid antibody at 0.1 µg/ml dilution.
