ARG56970
anti-Bid antibody [4D3]
anti-Bid antibody [4D3] for ICC/IF,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody
概述
| 产品描述 | Mouse Monoclonal antibody [4D3] recognizes Bid |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 4D3 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Bid |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-195 of Human Bid. |
| 偶联标记 | Un-conjugated |
| 別名 | p22 BID; BID; p15 BID; p11 BID; BH3-interacting domain death agonist; p13 BID; FP497 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P55957 Human BH3-interacting domain death agonist |
|---|---|
| 基因名称 | BID |
| 全名 | BH3 interacting domain death agonist |
| 背景介绍 | This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008] |
| 生物功能 | The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody |
| 预测分子量 | 22 kDa |
| 翻译后修饰 | TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products. p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation. |
检测图片 (2) Click the Picture to Zoom In
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ARG56970 anti-Bid antibody [4D3] ICC/IF image
Immunoflorescense: HeLa cell line stained with ARG56970 anti-Bid antibody [4D3] at 1:100 (green).
DAPI (Blue) for nucleus staining.
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ARG56970 anti-Bid antibody [4D3] WB image
Western blot: 20 µg of Jurkat stained with ARG56970 anti-Bid antibody [4D3] at 1:1000.
