ARG57142
anti-Beclin 1 antibody [45E8]
anti-Beclin 1 antibody [45E8] for Flow cytometry,ICC/IF,Western blot and Human
Cancer antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Autophagy Study antibody
概述
| 产品描述 | Mouse Monoclonal antibody [45E8] recognizes Beclin 1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 45E8 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | Beclin 1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-450 of Human Beclin-1 |
| 偶联标记 | Un-conjugated |
| 別名 | BECN1; Beclin 1; VPS30; ATG6; Coiled-Coil Myosin-Like BCL2-Interacting Protein; Beclin 1, Autophagy Related; Beclin-1; Beclin 1 (Coiled-Coil, Moesin-Like BCL2 Interacting Protein); Beclin 1 (Coiled-Coil, Moesin-Like BCL2-Interacting Protein); ATG6 Autophagy Related 6 Homolog (S. Cerevisiae); Testis Secretory Sperm-Binding Protein Li 215e; ATG6 Autophagy Related 6 Homolog; Beclin1-Gamma; Protein GT197; Beclin1-Beta; Beclin1; GT197 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BECN1 |
| 全名 | beclin 1, autophagy related |
| 背景介绍 | This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| 生物功能 | Protects against infection by a neurovirulent strain of Sindbis virus. May play a role in antiviral host defense. [UniProt] |
| 细胞定位 | Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Mitochondrion, Nucleus. [UniProt] |
| 产品亮点 | Related products: Beclin 1 antibodies; Beclin 1 Duos / Panels; |
| 研究领域 | Cancer antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Autophagy Study antibody |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG57142 anti-Beclin 1 antibody [45E8] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57142 anti-Beclin 1 antibody [45E8] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57142 anti-Beclin 1 antibody [45E8] FACS image
Flow Cytometry: HeLa cell line stained with ARG57142 anti-Beclin 1 antibody [45E8] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57142 anti-Beclin 1 antibody [45E8] WB image
Western blot: 10 ng of 1) Recombinant Human BECN1 protein, and 2) Balb/3T3 cell lysates stained with ARG57142 anti-Beclin 1 antibody [45E8] at 1:5000.
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ARG57142 anti-Beclin 1 antibody [45E8] WB image
Western blot: 10 µg of 1) 293T, and 2) BECN1 Transfected 293T cell lysate stained with ARG57142 anti-Beclin 1 antibody [45E8] at 1:1000.
