ARG51692
anti-Bcr phospho (Tyr177) antibody
anti-Bcr phospho (Tyr177) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Bcr phospho (Tyr177) |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Bcr |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of tyrosine 177 (P-F-Y(p)-V-N) derived from Human Bcr. |
| 偶联标记 | Un-conjugated |
| 別名 | ALL; CML; Renal carcinoma antigen NY-REN-26; D22S11; PHL; D22S662; BCR1; EC 2.7.11.1; Breakpoint cluster region protein |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BCR |
| 全名 | breakpoint cluster region |
| 背景介绍 | GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity. |
| 生物功能 | GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity. [UniProt] |
| 研究领域 | Cancer antibody; Signaling Transduction antibody |
| 预测分子量 | 143 kDa |
| 翻译后修饰 | Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2. |
检测图片 (2) Click the Picture to Zoom In
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ARG51692 anti-Bcr phospho (Tyr177) antibody WB image
Western blot: Extracts from K562 cells untreated or treated with H2O2 stained with ARG51692 anti-Bcr phospho (Tyr177) antibody.
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ARG51692 anti-Bcr phospho (Tyr177) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tumor tissue stained with ARG51692 anti-Bcr phospho (Tyr177) antibody (left) or the same antibody preincubated with blocking peptide (right).
