ARG51569
anti-Bcl XL phospho (Ser62) antibody
anti-Bcl XL phospho (Ser62) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Bcl XL phospho (Ser62) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Bcl XL |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of serine 62(A-D-S(p)-P-A) derived from Human BCL-XL . |
| 偶联标记 | Un-conjugated |
| 別名 | Apoptosis regulator Bcl-X; BCLXS; BCL-XL/S; PPP1R52; bcl-xS; Bcl-2-like protein 1; Bcl2-L-1; Bcl-X; BCLX; bcl-xL; BCL2L; BCLXL |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BCL2L1 |
| 全名 | BCL2-like 1 |
| 背景介绍 | Potent inhibitor of cell death. Inhibits activation of caspases . Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(S) promotes apoptosis |
| 生物功能 | Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. Isoform Bcl-X(S) promotes apoptosis. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30233 Phospho Bcl XL Antibody Duo (Total, pS62) Related products: Bcl XL antibodies; Bcl XL Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 26 kDa |
| 翻译后修饰 | Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis. |
检测图片 (4) Click the Picture to Zoom In
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ARG51569 anti-Bcl XL phospho (Ser62) antibody WB image
Western blot: Extracts from HeLa cells untreated or treated with EGF stained with ARG51569 anti-Bcl XL phospho (Ser62) antibody.
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ARG51569 anti-Bcl XL phospho (Ser62) antibody WB image
Western blot: Extracts from HeLa cells, treated with Noc or calf intestinal phosphatase (CIP), stained with ARG51569 anti-Bcl XL phospho (Ser62) antibody.
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ARG51569 anti-Bcl XL phospho (Ser62) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51569 anti-Bcl XL phospho (Ser62) antibody (red).
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ARG51569 anti-Bcl XL phospho (Ser62) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51569 anti-Bcl XL phospho (Ser62) antibody.
