ARG54723
anti-Bcl 2 phospho (Ser70) antibody
anti-Bcl 2 phospho (Ser70) antibody for Dot blot,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Bcl 2 phospho (Ser70) |
|---|---|
| 反应物种 | Hu |
| 应用 | Dot, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Bcl 2 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated phosphospecific peptide around Ser70 of Human Bcl 2 (NP_000624.2). |
| 偶联标记 | Un-conjugated |
| 別名 | BCL2; BCL2 Apoptosis Regulator; PPP1R50; Bcl-2; Protein Phosphatase 1, Regulatory Subunit 50; Apoptosis Regulator Bcl-2; B-Cell CLL/Lymphoma 2; BCL2, Apoptosis Regulator |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Jurkat + Paclitaxel |
属性
| 纯化 | Protein A purified |
|---|---|
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BCL2 |
| 全名 | B-cell CLL/lymphoma 2 |
| 背景介绍 | This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| 生物功能 | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. [UniProt] |
| 细胞定位 | Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30268 Apoptosis Marker Antibody Duo (Bcl2, Bax) ARG30269 Apoptosis Marker Antibody Duo (Bcl2, Bid) Related products: Bcl-2 antibodies; Bcl-2 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Lymphoma |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody |
| 预测分子量 | 26 kDa |
| 翻译后修饰 | Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, which stimulates starvation-induced autophag [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG54723 anti-Bcl 2 phospho (Ser70) antibody WB image
Western blot: Extracts from Jurkat cells, treated or untreated with Paclitaxel. The blots were stained with ARG54723 anti-Bcl 2 phospho (Ser70) antibody.
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ARG54723 anti-Bcl 2 phospho (Ser70) antibody Dot image
Dot blot: Analysis of ARG54723 anti-Bcl 2 phospho (Ser70) antibody on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6 µg/ml.
