ARG57102
anti-Bak antibody [38E2]
anti-Bak antibody [38E2] for ICC/IF,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Cell Death antibody; Pro-apoptotic Bcl2 protein antibody
概述
产品描述 | Mouse Monoclonal antibody [38E2] recognizes Bak |
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反应物种 | Hu, Ms |
应用 | ICC/IF, WB |
宿主 | Mouse |
克隆 | Monoclonal |
克隆号 | 38E2 |
同位型 | IgG2a, kappa |
靶点名称 | Bak |
抗原物种 | Human |
抗原 | Recombinant fragment around aa. 29-187 of Human Bak |
偶联标记 | Un-conjugated |
別名 | Apoptosis regulator BAK; BCL2L7; Bcl-2-like protein 7; CDN1; Bcl2-L-7; Bcl-2 homologous antagonist/killer; BAK-LIKE; BAK |
应用说明
应用建议 |
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应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Purification with Protein G. |
缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 10% Glycerol |
浓度 | 1 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 |
Swiss-port # O08734 Mouse Bcl-2 homologous antagonist/killer Swiss-port # Q16611 Human Bcl-2 homologous antagonist/killer |
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基因名称 | BAK1 |
全名 | BCL2-antagonist/killer 1 |
背景介绍 | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008] |
生物功能 | In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. [UniProt] |
研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody; Pro-apoptotic Bcl2 protein antibody |
预测分子量 | 23 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG57102 anti-Bak antibody [8B4] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57102 anti-Bak antibody [8B4] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57102 anti-Bak antibody [8B4] FACS image
Flow Cytometry: HeLa cell line stained with ARG57102 anti-Bak antibody [8B4] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57102 anti-Bak antibody [8B4] WB image
Western blot: 40 µg of 1) 293T, 2) HeLa, 3) A431, 4) A549, 5) Jurkat, 6) MCF7, and 7) PC3 cell lysates stained with ARG57102 anti-Bak antibody [8B4] at 1:1000.
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ARG57102 anti-Bak antibody [8B4] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG57102 anti-Bak antibody [8B4] at 1:500 (Red).
Hoechst 3342 (Blue) for nucleus staining.