ARG57102

anti-Bak antibody [38E2]

anti-Bak antibody [38E2] for ICC/IF,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody; Cell Death antibody; Pro-apoptotic Bcl2 protein antibody

概述

产品描述 Mouse Monoclonal antibody [38E2] recognizes Bak
反应物种 Hu, Ms
应用 ICC/IF, WB
宿主 Mouse
克隆 Monoclonal
克隆号 38E2
同位型 IgG2a, kappa
靶点名称 Bak
抗原物种 Human
抗原 Recombinant fragment around aa. 29-187 of Human Bak
偶联标记 Un-conjugated
別名 Apoptosis regulator BAK; BCL2L7; Bcl-2-like protein 7; CDN1; Bcl2-L-7; Bcl-2 homologous antagonist/killer; BAK-LIKE; BAK

应用说明

应用建议
应用 推荐稀释比
ICC/IFAssay-dependent
WB1:250 - 1:500
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 10% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 12018 Mouse BAK1

GeneID: 578 Human BAK1

Swiss-port # O08734 Mouse Bcl-2 homologous antagonist/killer

Swiss-port # Q16611 Human Bcl-2 homologous antagonist/killer

基因名称 BAK1
全名 BCL2-antagonist/killer 1
背景介绍 The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008]
生物功能 In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. [UniProt]
研究领域 Cell Biology and Cellular Response antibody; Cell Death antibody; Pro-apoptotic Bcl2 protein antibody
预测分子量 23 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG57102 anti-Bak antibody [8B4] ICC/IF image

    Immunofluorescence: HeLa cells line stained with ARG57102 anti-Bak antibody [8B4] at 1:100 (Green).

    DAPI (Blue) for nucleus staining.

  • ARG57102 anti-Bak antibody [8B4] FACS image

    Flow Cytometry: HeLa cell line stained with ARG57102 anti-Bak antibody [8B4] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).

  • ARG57102 anti-Bak antibody [8B4] WB image

    Western blot: 40 µg of 1) 293T, 2) HeLa, 3) A431, 4) A549, 5) Jurkat, 6) MCF7, and 7) PC3 cell lysates stained with ARG57102 anti-Bak antibody [8B4] at 1:1000.

  • ARG57102 anti-Bak antibody [8B4] ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG57102 anti-Bak antibody [8B4] at 1:500 (Red).

    Hoechst 3342 (Blue) for nucleus staining.