ARG59190
anti-BTG2 antibody
anti-BTG2 antibody for Flow cytometry,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes BTG2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | BTG2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence of Human BTG2. (EQRLKVFSGALQEALTEHYKHHWFPEK) |
| 偶联标记 | Un-conjugated |
| 別名 | PC3; Protein BTG2; BTG family member 2; TIS21; NGF-inducible anti-proliferative protein PC3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BTG2 |
| 全名 | BTG family, member 2 |
| 背景介绍 | The protein encoded by this gene is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. This encoded protein is involved in the regulation of the G1/S transition of the cell cycle. [provided by RefSeq, Jul 2008] |
| 生物功能 | Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex. Activates mRNA deadenylation in a CNOT6 and CNOT7-dependent manner. In vitro can inhibit deadenylase activity of CNOT7 and CNOT8. Involved in cell cycle regulation. Could be involved in the growth arrest and differentiation of the neuronal precursors (By similarity). Modulates transcription regulation mediated by ESR1. Involved in mitochondrial depolarization and neurite outgrowth. [UniProt] |
| 预测分子量 | 17 kDa |
| 翻译后修饰 | Phosphorylated at Ser-147 by MAPK1/ERK2 and MAPK3/ERK1, and at Ser-149 by MAPK14, leading to PIN1-binding and mitochondrial depolarization. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG59190 anti-BTG2 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat brain, Rat brain, Rat kidney, Mouse brain, Mouse brain, Mouse kidney, MDA-MB-231 and MDA-MB-453 whole cell lysates stained with ARG59190 anti-BTG2 antibody at 0.5 µg/ml, overnight at 4°C.
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ARG59190 anti-BTG2 antibody FACS image
Flow Cytometry: U2OS cells were blocked with 10% normal goat serum and then stained with ARG59190 anti-BTG2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
