ARG43192
anti-BRCC36 antibody
anti-BRCC36 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes BRCC36 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | BRCC36 |
| 抗原物种 | Human |
| 抗原 | A 18-amino acid peptide within aa. 20-70 of Human BRCC36. |
| 偶联标记 | Un-conjugated |
| 別名 | BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36; BRCC36; CXorf53; BRCA1/BRCA2-containing complex subunit 3; Lys-63-specific deubiquitinase BRCC36; EC 3.4.19.-; C6.1A; BRCA1-A complex subunit BRCC36 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | MCF7 | ||||||||
| 实际分子量 | ~ 35 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P46736 Human Lys-63-specific deubiquitinase BRCC36 |
|---|---|
| 基因名称 | BRCC3 |
| 全名 | BRCA1/BRCA2-containing complex, subunit 3 |
| 背景介绍 | This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jun 2011] |
| 生物功能 | Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed:19214193, PubMed:20656690, PubMed:24075985, PubMed:26344097). Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) (PubMed:20656690). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:20656690, PubMed:24075985, PubMed:26344097, PubMed:26195665). Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex (PubMed:19214193). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985, PubMed:26344097). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985). [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Note=Localizes at sites of DNA damage at double-strand breaks. Interaction with ABRAXAS2 retains BRCC3 in the cytoplasm. [UniProt] |
| 预测分子量 | 36 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG43192 anti-BRCC36 antibody ICC/IF image
Immunofluorescence: Human brain cells stained with ARG43192 anti-BRCC36 antibody at 20 µg/ml dilution.
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ARG43192 anti-BRCC36 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG43192 anti-BRCC36 antibody at 2.5 µg/ml dilution.
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ARG43192 anti-BRCC36 antibody WB image
Western blot: MCF7 cell lysate stained with ARG43192 anti-BRCC36 antibody at 0.5 and 1 µg/ml dilution.
