ARG66736
anti-BLM / Blooms Syndrome Protein antibody
anti-BLM / Blooms Syndrome Protein antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes BLM / Blooms Syndrome Protein |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | BLM / Blooms Syndrome Protein |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide between aa. 65-114 of Human BLM / Blooms Syndrome Protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Bloom syndrome protein; RECQL3; RECQL2; RECQ2; BS; RecQ protein-like 3; EC 3.6.4.12; DNA helicase, RecQ-like type 2; RecQ2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BLM |
| 全名 | Bloom syndrome, RecQ helicase-like |
| 背景介绍 | The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008] |
| 生物功能 | ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction (PubMed:9388193, PubMed:24816114, PubMed:25901030). Participates in DNA replication and repair (PubMed:12019152, PubMed:21325134, PubMed:23509288). Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed:21325134). Negatively regulates sister chromatid exchange (SCE) (PubMed:25901030). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution (PubMed:25901030). Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction (PubMed:20639533, PubMed:24257077, PubMed:25901030). [UniProt] |
| 细胞定位 | Nucleus. Note=Together with SPIDR, is redistributed in discrete nuclear DNA damage-induced foci following hydroxyurea (HU) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a RMI complex- and SPIDR-dependent manner. [UniProt] |
| 预测分子量 | 159 kDa |
| 翻译后修饰 | Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG66736 anti-BLM / Blooms Syndrome Protein antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG66736 anti-BLM / Blooms Syndrome Protein antibody. The picture on the right is blocked with the synthetic peptide.
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ARG66736 anti-BLM / Blooms Syndrome Protein antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lymph node tissue stained with ARG66736 anti-BLM / Blooms Syndrome Protein antibody. The picture on the right is blocked with the synthetic peptide.
