ARG46046

anti-BIT1 antibody

anti-BIT1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

概述

产品描述 Rabbit Polyclonal antibody recognizes BIT1
反应物种 Hu, Ms
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 BIT1
抗原物种 Human
抗原 A 16 amino acid synthetic peptide within aa. 80 - 130 of human BIT1.
偶联标记 Un-conjugated
別名 PTRH2; Bit1; BIT1; PTH2; CGI-147; BIT1; Peptidyl-tRNA hydrolase 2; mitochondrial; Bcl-2 inhibitor of transcription 1; PTH 2

应用说明

应用建议
应用 推荐稀释比
IHC-PAssay-dependent
WBAssay-dependent
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

纯化 Affinity chromatography purified
缓冲液 PBS and 0.02% Sodium azide.
抗菌剂 0.02% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -96°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 217057 Mouse PTRH2

GeneID: 51651 Human PTRH2

Swiss-port # Q8R2Y8 Mouse Peptidyl-tRNA hydrolase 2, mitochondrial

Swiss-port # Q9Y3E5 Human Peptidyl-tRNA hydrolase 2, mitochondrial

基因名称 PTRH2
全名 peptidyl-tRNA hydrolase 2
背景介绍 The protein encoded by this gene is a mitochondrial protein with two putative domains, an N-terminal mitochondrial localization sequence, and a UPF0099 domain. In vitro assays suggest that this protein possesses peptidyl-tRNA hydrolase activity, to release the peptidyl moiety from tRNA, thereby preventing the accumulation of dissociated peptidyl-tRNA that could reduce the efficiency of translation. This protein also plays a role regulating cell survival and death. It promotes survival as part of an integrin-signaling pathway for cells attached to the extracellular matrix (ECM), but also promotes apoptosis in cells that have lost their attachment to the ECM, a process called anoikos. After loss of cell attachment to the ECM, this protein is phosphorylated, is released from the mitochondria into the cytosol, and promotes caspase-independent apoptosis through interactions with transcriptional regulators. This gene has been implicated in the development and progression of tumors, and mutations in this gene have been associated with an infantile multisystem neurologic, endocrine, and pancreatic disease (INMEPD) characterized by intellectual disability, postnatal microcephaly, progressive cerebellar atrophy, hearing impairment, polyneuropathy, failure to thrive, and organ fibrosis with exocrine pancreas insufficiency (PMID: 25574476). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]
生物功能 Peptidyl-tRNA hydrolase which releases tRNAs from the ribosome during protein synthesis (PubMed:14660562). Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1. [UniProt]
细胞定位 Mitochondrion outer membrane. [UniProt]
预测分子量 19 kDa
翻译后修饰 Phosphoprotein. [UniProt]