ARG64302

anti-BHMT antibody

anti-BHMT antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Metabolism antibody; Signaling Transduction antibody

概述

产品描述 Goat Polyclonal antibody recognizes BHMT
反应物种 Hu, Ms, Rat
预测物种 Dog
应用 IHC-P, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 BHMT
抗原物种 Human
抗原 C-EQQLKELFEKQK
偶联标记 Un-conjugated
別名 HEL-S-61p; Betaine--homocysteine S-methyltransferase 1; EC 2.1.1.5; BHMT1

应用说明

应用建议
应用 推荐稀释比
IHC-P5 µg/ml
WB0.03 - 0.1 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 12116 Mouse BHMT

GeneID: 635 Human BHMT

GeneID: 81508 Rat BHMT

背景介绍 This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]
研究领域 Metabolism antibody; Signaling Transduction antibody
预测分子量 45 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG64302 anti-BHMT antibody WB image

    Western Blot: Rat Liver lysate (35 µg protein in RIPA buffer) stained with ARG64302 anti-BHMT antibody at 0.03 µg/ml dilution.

  • ARG64302 anti-BHMT antibody WB image

    Western Blot: 1). Mock transfection; 2) BHMT RC203148) expressing plasmid transfected HEK293 cell lysate standed with ARG64302 anti-BHMT antibody

  • ARG64302 anti-BHMT antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64302 anti-BHMT antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG64302 anti-BHMT antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64302 anti-BHMT antibody at 5 µg/ml dilution followed by AP-staining.