ARG56932
anti-BHMT antibody [3D6]
anti-BHMT antibody [3D6] for Flow cytometry,ICC/IF,Western blot and Human,Mouse
概述
| 产品描述 | Mouse Monoclonal antibody [3D6] recognizes BHMT |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3D6 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | BHMT |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-406 of Human BHMT. |
| 偶联标记 | Un-conjugated |
| 別名 | HEL-S-61p; Betaine--homocysteine S-methyltransferase 1; EC 2.1.1.5; BHMT1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O35490 Mouse Betaine--homocysteine S-methyltransferase 1 Swiss-port # Q93088 Human Betaine--homocysteine S-methyltransferase 1 |
|---|---|
| 基因名称 | BHMT |
| 全名 | betaine--homocysteine S-methyltransferase |
| 背景介绍 | This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008] |
| 生物功能 | Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline. [UniProt] |
| 预测分子量 | 45 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG56932 anti-BHMT antibody [3D6] ICC/IF image
Immunoflorescense: Hep3B cell line stained with ARG56932 anti-BHMT antibody [3D6] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG56932 anti-BHMT antibody [3D6] FACS image
Flow Cytometry: Hep3B cell line stained with ARG56932 anti-BHMT antibody [3D6] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.
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ARG56932 anti-BHMT antibody [3D6] WB image
Western blot: 20 µg of Mouse liver stained with ARG56932 anti-BHMT antibody [3D6] at 1:1000.
