ARG65673
anti-BDNF antibody
anti-BDNF antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
参考文献3
概述
| 产品描述 | Goat Polyclonal antibody recognizes BDNF |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Dog, Rat |
| 应用 | ICC/IF, IHC-Fr, IHC-P, WB |
| 特异性 | This antibody is expected to recognise all reported isoforms (NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1) |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 靶点名称 | BDNF |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the internal region of Human BDNF (ETKCNPMGYTKE) |
| 偶联标记 | Un-conjugated |
| 別名 | Brain-derived neurotrophic factor; Abrineurin; BULN2; ANON2; BDNF |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: WB was tested by the publication (dio: 10.21203/rs.3.rs-929317/v1) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BDNF |
| 全名 | brain-derived neurotrophic factor |
| 背景介绍 | The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009] |
| 生物功能 | During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. [UniProt] |
| 产品亮点 | Related products: BDNF antibodies; BDNF ELISA Kits; BDNF recombinant proteins; Related news: The role of HDGF in tumor angiogenesis |
| 研究领域 | Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody |
| 预测分子量 | 28 kDa |
| 翻译后修饰 | The propeptide is N-glycosylated and glycosulfated. Converted into mature BDNF by plasmin (PLG). |
检测图片 (6) Click the Picture to Zoom In
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ARG65673 anti-BDNF antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton. Cells were stained with ARG65673 anti-BDNF antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG65673 anti-BDNF antibody IHC-Fr image
Immunohistochemistry: PFA-perfused cryosection of Mouse sciatic nerve tissue stained with ARG65673 anti-BDNF antibody at 1 µg/ml dilution.
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ARG65673 anti-BDNF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65673 anti-BDNF antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG65673 anti-BDNF antibody IHC image
Immunohistochemistry: paraffin-embedded Rat Cerebral Cortex (A) and Rat Hippocampus (B) stained with ARG65673 anti-BDNF antibody (red) at 5 ug/ml dilution. CRY-staining with DAPI nuclear counter stain in blue. Antigen retrieval with citrate buffer pH 6 at 95-98°C.
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ARG65673 anti-BDNF antibody IHC image
Immunohistochemistry: paraffin-embedded Rat Cerebral Cortex stained with ARG65673 anti-BDNF antibody at 5 ug/ml dilution. Antigen retrieval with citrate buffer pH 6 at 95-98°C.
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ARG65673 anti-BDNF antibody IHC-Fr image
Immunohistochemistry: PFA-perfused cryosection of Mouse injured sciatic nerve tissue stained with ARG65673 anti-BDNF antibody at 1 µg/ml dilution.
文献引用
G721-0282 Exerts Anxiolytic-Like Effects on Chronic Unpredictable Mild Stress in Mice Through Inhibition of Chitinase-3-Like 1-Mediated Neuroinflammation
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Neuroprotective Effects of Estrogen Through BDNF-Transient Receptor Potential Channels 6 Signaling Pathway in the Hippocampus in a Rat Model of Perimenopausal Depression
function /
p53 Knock Out Mice Enhances Anxiety- and Depression-Like Behaviors through an Increase of Glutamate, Calcium, and Cytokine-Mediated Cell Death Signals
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