ARG65673

anti-BDNF antibody

anti-BDNF antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
publication_link 参考文献3

概述

产品描述 Goat Polyclonal antibody recognizes BDNF
反应物种 Hu, Ms
预测物种 Dog, Rat
应用 ICC/IF, IHC-Fr, IHC-P, WB
特异性 This antibody is expected to recognise all reported isoforms (NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1)
宿主 Goat
克隆 Polyclonal
靶点名称 BDNF
抗原物种 Human
抗原 Synthetic peptide around the internal region of Human BDNF (ETKCNPMGYTKE)
偶联标记 Un-conjugated
別名 Brain-derived neurotrophic factor; Abrineurin; BULN2; ANON2; BDNF

应用说明

应用建议
应用 推荐稀释比
ICC/IF10 µg/ml
IHC-Fr1-3 µg/ml
IHC-PAssay - dependent
WBAssay dependent
应用说明 IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: WB was tested by the publication (dio: 10.21203/rs.3.rs-929317/v1)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purified
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 12064 Mouse BDNF

GeneID: 627 Human BDNF

Swiss-port # P21237 Mouse Brain-derived neurotrophic factor

Swiss-port # P23560 Human Brain-derived neurotrophic factor

基因名称 BDNF
全名 brain-derived neurotrophic factor
背景介绍 The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009]
生物功能 During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. [UniProt]
产品亮点 Related products:
BDNF antibodies; BDNF ELISA Kits; BDNF recombinant proteins;
Related news:
The role of HDGF in tumor angiogenesis
研究领域 Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
预测分子量 28 kDa
翻译后修饰 The propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG).

检测图片 (6) Click the Picture to Zoom In

  • ARG65673 anti-BDNF antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton. Cells were stained with ARG65673 anti-BDNF antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

  • ARG65673 anti-BDNF antibody IHC-Fr image

    Immunohistochemistry: PFA-perfused cryosection of Mouse sciatic nerve tissue stained with ARG65673 anti-BDNF antibody at 1 µg/ml dilution.

  • ARG65673 anti-BDNF antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65673 anti-BDNF antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG65673 anti-BDNF antibody IHC image

    Immunohistochemistry: paraffin-embedded Rat Cerebral Cortex (A) and Rat Hippocampus (B) stained with ARG65673 anti-BDNF antibody (red) at 5 ug/ml dilution. CRY-staining with DAPI nuclear counter stain in blue. Antigen retrieval with citrate buffer pH 6 at 95-98°C.

  • ARG65673 anti-BDNF antibody IHC image

    Immunohistochemistry: paraffin-embedded Rat Cerebral Cortex stained with ARG65673 anti-BDNF antibody at 5 ug/ml dilution. Antigen retrieval with citrate buffer pH 6 at 95-98°C.

  • ARG65673 anti-BDNF antibody IHC-Fr image

    Immunohistochemistry: PFA-perfused cryosection of Mouse injured sciatic nerve tissue stained with ARG65673 anti-BDNF antibody at 1 µg/ml dilution.

文献引用

G721-0282 Exerts Anxiolytic-Like Effects on Chronic Unpredictable Mild Stress in Mice Through Inhibition of Chitinase-3-Like 1-Mediated Neuroinflammation

WB / Mouse

Hyeon Joo Ham et al.
Front Cell Neurosci,  (2022)

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Neuroprotective Effects of Estrogen Through BDNF-Transient Receptor Potential Channels 6 Signaling Pathway in the Hippocampus in a Rat Model of Perimenopausal Depression

function / 

Qiaoli Song et al.
Front Aging Neurosci.,  (2022)

publication_link

 

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p53 Knock Out Mice Enhances Anxiety- and Depression-Like Behaviors through an Increase of Glutamate, Calcium, and Cytokine-Mediated Cell Death Signals

WB / Mouse

In Jun Yeo  et al.
Research Square,  (2021)

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