ARG58201
anti-BDNF antibody
anti-BDNF antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes BDNF |
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| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | BDNF |
| 抗原物种 | Human |
| 抗原 | Partial recombinant protein corresponding to aa. 129-247 of Human BDNF. |
| 偶联标记 | Un-conjugated |
| 別名 | Brain-derived neurotrophic factor; Abrineurin; BULN2; ANON2; BDNF |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min followed by cooling at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 28 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.025% Sodium azide and 2.5% BSA. |
| 抗菌剂 | 0.025% Sodium azide |
| 稳定剂 | 2.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BDNF |
| 全名 | brain-derived neurotrophic factor |
| 背景介绍 | The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009] |
| 生物功能 | During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. [UniProt] |
| 细胞定位 | Cytoplasmic, secreted. [UniProt] |
| 产品亮点 | Related products: BDNF antibodies; BDNF ELISA Kits; BDNF recombinant proteins; Anti-Rabbit IgG secondary antibodies; Related news: The role of HDGF in tumor angiogenesis |
| 预测分子量 | 28 kDa |
| 翻译后修饰 | The propeptide is N-glycosylated and glycosulfated. Converted into mature BDNF by plasmin (PLG). [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG58201 anti-BDNF antibody FACS image
Flow Cytometry: U-87 MG cells stained with ARG58201 anti-BDNF antibody at 1 µg/10^6 cells (blocked with Goat sera). Red: Cells alone, Green: Isotype control, Blue: Primary antibody.
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ARG58201 anti-BDNF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain tissue stained with ARG58201 anti-BDNF antibody. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min followed by cooling at RT.
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ARG58201 anti-BDNF antibody WB image
Western blot: Rat brain lysate stained with ARG58201 anti-BDNF antibody at 0.5 µg/ml dilution.
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ARG58201 anti-BDNF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue stained with ARG58201 anti-BDNF antibody. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min followed by cooling at RT.
