ARG43129
anti-BCL9L antibody
anti-BCL9L antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes BCL9L |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | BCL9L |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to E397-A666 of Human BCL9L. |
| 偶联标记 | Un-conjugated |
| 別名 | Protein BCL9-2; BCL9-2; B-cell CLL/lymphoma 9-like protein; DLNB11; BCL9-like protein; B-cell lymphoma 9-like protein |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunogen affinity purified. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q67FY2 Mouse B-cell CLL/lymphoma 9-like protein Swiss-port # Q86UU0 Human B-cell CLL/lymphoma 9-like protein |
|---|---|
| 基因名称 | BCL9L |
| 全名 | B-cell CLL/lymphoma 9-like |
| 生物功能 | Transcriptional regulator that acts as an activator. Promotes beta-catenin transcriptional activity. Plays a role in tumorigenesis. Enhances the neoplastic transforming activity of CTNNB1 (By similarity). [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 157 kDa |
检测图片 (9) Click the Picture to Zoom In
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ARG43129 anti-BCL9L antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG43129 anti-BCL9L antibody (green) at 2 µg/ml dilution, overnight at 4°C.
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ARG43129 anti-BCL9L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43129 anti-BCL9L antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43129 anti-BCL9L antibody FACS image
Flow Cytometry: SiHa cells were blocked with 10% normal goat serum and then stained with ARG43129 anti-BCL9L antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG43129 anti-BCL9L antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG43129 anti-BCL9L antibody (green) at 2 µg/ml dilution, overnight at 4°C.
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ARG43129 anti-BCL9L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43129 anti-BCL9L antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43129 anti-BCL9L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43129 anti-BCL9L antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43129 anti-BCL9L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human oesophagus squama cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43129 anti-BCL9L antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43129 anti-BCL9L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43129 anti-BCL9L antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43129 anti-BCL9L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43129 anti-BCL9L antibody at 1 µg/ml dilution, overnight at 4°C.
