ARG45551
anti-BCKDHA antibody
anti-BCKDHA antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes BCKDHA |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | BCKDHA |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human BCKDHA. |
| 偶联标记 | Un-conjugated |
| 別名 | BCKDHA; Branched Chain Keto Acid Dehydrogenase E1 Subunit Alpha; 2-Oxoisovalerate Dehydrogenase Subunit Alpha, Mitochondrial; MSU; Branched-Chain Alpha-Keto Acid Dehydrogenase E1 Component Alpha Chain; Branched Chain Keto Acid Dehydrogenase E1, Alpha Polypeptide; 2-Oxoisovalerate Dehydrogenase (Lipoamide); BCKDH E1-Alpha; EC 1.2.4.4; BCKDE1A; OVD1A; Branched Chain Keto Acid Dehydrogenase E1, Alpha Polypeptide (Maple Syrup Urine Disease); Branched Chain Keto Acid Dehydrogenase E1 Alpha Protein; Maple Syrup Urine Disease; MSUD1A; MSUD1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 50 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P11960 Rat 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial Swiss-port # P12694 Human 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial |
|---|---|
| 基因名称 | BCKDHA |
| 全名 | Branched Chain Keto Acid Dehydrogenase E1 Subunit Alpha |
| 背景介绍 | The branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). This gene encodes the alpha subunit of the decarboxylase (E1) component. Mutations in this gene result in maple syrup urine disease, type IA. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
| 生物功能 | Together with BCKDHB forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction. [UniProt] |
| 细胞定位 | Mitochondrion. [UniProt] |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Acetylation; Phosphoprotein. [UniProt] |
检测图片 (7) Click the Picture to Zoom In
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ARG45551 anti-BCKDHA antibody IHC-P image
Immunohistochemistry: Human liver cancer stained with ARG45551 anti-BCKDHA antibody at 2 μg/ml dilution.
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ARG45551 anti-BCKDHA antibody ICC/IF image
Immunofluorescence: A549 stained with ARG45551 anti-BCKDHA antibody at 5 μg/ml dilution.
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ARG45551 anti-BCKDHA antibody WB image
Western blot: MCF-7 stained with ARG45551 anti-BCKDHA antibody at 0.5 μg/ml dilution.
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ARG45551 anti-BCKDHA antibody WB image
Western blot: Rat testis stained with ARG45551 anti-BCKDHA antibody at 0.5 μg/ml dilution.
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ARG45551 anti-BCKDHA antibody FACS image
Flow Cytometry: PC-3 stained with ARG45551 anti-BCKDHA antibody at 1 µg/10^6 cells dilution.
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ARG45551 anti-BCKDHA antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG45551 anti-BCKDHA antibody at 2 μg/ml dilution.
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ARG45551 anti-BCKDHA antibody IHC-P image
Immunohistochemistry: Mouse brain stained with ARG45551 anti-BCKDHA antibody at 2 μg/ml dilution.
