ARG41252
anti-BCAR1 / p130 Cas antibody [M144]
anti-BCAR1 / p130 Cas antibody [M144] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [M144] recognizes BCAR1 / p130 Cas |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Chk |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | M144 |
| 同位型 | IgG1 |
| 靶点名称 | BCAR1 / p130 Cas |
| 抗原物种 | Rat |
| 抗原 | Recombinant protein around the C-terminus of Rat BCAR1 / p130 Cas. This sequence is highly conserved in Human, Mouse and Chicken. |
| 偶联标记 | Un-conjugated |
| 別名 | CASS1; CAS; p130cas; P130Cas; Cas scaffolding protein family member 1; Breast cancer anti-estrogen resistance protein 1; CAS1; CRK-associated substrate; CRKAS |
应用说明
| 应用建议 |
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| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 130 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 50% Glycerol and 1 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P56945 Human Breast cancer anti-estrogen resistance protein 1 |
|---|---|
| 基因名称 | BCAR1 |
| 全名 | breast cancer anti-estrogen resistance 1 |
| 背景介绍 | BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009] |
| 生物功能 | Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells. [UniProt] |
| 细胞定位 | Cell junction, focal adhesion. Cytoplasm. Note=Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation. [UniProt] |
| 预测分子量 | 93 kDa |
| 翻译后修饰 | PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix. Dephosphorylated by PTPN14 at Tyr-128. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG41252 anti-BCAR1 / p130 Cas antibody [M144] WB image
Western blot: Human endothelial cells serum starved overnight (left) or treated with pervanadate (1 mM) for 30 minutes (right). The blots were stained with ARG41252 anti-BCAR1 / p130 Cas antibody [M144].
