ARG45528
anti-BAZ1A / ACF1 antibody
anti-BAZ1A / ACF1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat,Monkey
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes BAZ1A / ACF1 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Mk |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | BAZ1A / ACF1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human BAZ1A / ACF1. |
| 偶联标记 | Un-conjugated |
| 別名 | BAZ1A; bromodomain adjacent to zinc finger domain, 1A; WCRF180; hWALp1; ACF1; CHRAC subunit ACF1; hACF1; Bromodomain adjacent to zinc finger domain protein 1A; Williams syndrome transcription factor-related chromatin-remodeling factor 180; WALp1; ATP-utilizing chromatin assembly and remodeling factor 1; ATP-dependent chromatin-remodeling protein |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 203 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9NRL2 Human Bromodomain adjacent to zinc finger domain protein 1A |
|---|---|
| 基因名称 | BAZ1A |
| 全名 | bromodomain adjacent to zinc finger domain, 1A |
| 背景介绍 | The BAZ1A gene encodes the accessory subunit of the ATP-dependent chromatin assembly factor (ACF), a member of the ISWI ('imitation switch') family of chromatin remodeling complexes (summarized by Racki et al., 2009 [PubMed 20033039]).[supplied by OMIM, Apr 2010] |
| 生物功能 | Component of the ACF complex, an ATP-dependent chromatin remodeling complex, that regulates spacing of nucleosomes using ATP to generate evenly spaced nucleosomes along the chromatin. The ATPase activity of the complex is regulated by the length of flanking DNA. Also involved in facilitating the DNA replication process. BAZ1A is the accessory, non-catalytic subunit of the complex which can enhance and direct the process provided by the ATPase subunit, SMARCA5, probably through targeting pericentromeric heterochromatin in late S phase. Moves end-positioned nucleosomes to a predominantly central position. May have a role in nuclear receptor-mediated transcription repression. Component of the histone-fold protein complex CHRAC complex which faciliates nucleosome sliding by the ACF complex and enhances ACF-mediated chromatin assembly. The C-terminal regions of both CHRAC1 and POLE1 are required for these functions. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 179 kDa |
| 翻译后修饰 | Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (6) Click the Picture to Zoom In
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ARG45528 anti-BAZ1A / ACF1 antibody ICC/IF image
Immunofluorescence: MCF-7 stained with ARG45528 anti-BAZ1A / ACF1 antibody at 5 μg/ml dilution.
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ARG45528 anti-BAZ1A / ACF1 antibody WB image
Western blot: Jurkat stained with ARG45528 anti-BAZ1A / ACF1 antibody at 0.5 μg/ml dilution.
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ARG45528 anti-BAZ1A / ACF1 antibody FACS image
Flow Cytometry: HepG2 stained with ARG45528 anti-BAZ1A / ACF1 antibody at 1 µg/10^6 cells dilution.
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ARG45528 anti-BAZ1A / ACF1 antibody WB image
Western blot: C6 stained with ARG45528 anti-BAZ1A / ACF1 antibody at 0.5 μg/ml dilution.
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ARG45528 anti-BAZ1A / ACF1 antibody WB image
Western blot: Mouse testis stained with ARG45528 anti-BAZ1A / ACF1 antibody at 0.5 μg/ml dilution.
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ARG45528 anti-BAZ1A / ACF1 antibody WB image
Western blot: COS-7 stained with ARG45528 anti-BAZ1A / ACF1 antibody at 0.5 μg/ml dilution.
