ARG63391
anti-BAG3 antibody
anti-BAG3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes BAG3 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | BAG3 |
| 抗原物种 | Human |
| 抗原 | C-SSMTDTPGNPAAP |
| 偶联标记 | Un-conjugated |
| 別名 | BAG-3; BIS; CAIR-1; Bcl-2-binding protein Bis; BAG family molecular chaperone regulator 3; MFM6; Docking protein CAIR-1; Bcl-2-associated athanogene 3 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O95817 Human BAG family molecular chaperone regulator 3 |
|---|---|
| 背景介绍 | BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody |
| 预测分子量 | 62 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG63391 anti-BAG3 antibody WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG63391 anti-BAG3 antibody at 1:1000 dilution.
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ARG63391 anti-BAG3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63391 anti-BAG3 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63391 anti-BAG3 antibody WB image
Western blot: 35 µg of MCF7 cell lysate (in RIPA buffer) stained with ARG63391 anti-BAG3 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
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ARG63391 anti-BAG3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63391 anti-BAG3 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63391 anti-BAG3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63391 anti-BAG3 antibody at 3.75 µg/ml dilution followed by AP-staining.
客户反馈
Excellent
anti-BAG3 antibody
Application：WB
Sample：293T
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
