ARG63391

anti-BAG3 antibody

anti-BAG3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
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概述

产品描述 Goat Polyclonal antibody recognizes BAG3
反应物种 Hu
应用 IHC-P, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 BAG3
抗原物种 Human
抗原 C-SSMTDTPGNPAAP
偶联标记 Un-conjugated
別名 BAG-3; BIS; CAIR-1; Bcl-2-binding protein Bis; BAG family molecular chaperone regulator 3; MFM6; Docking protein CAIR-1; Bcl-2-associated athanogene 3

应用说明

应用建议
应用 推荐稀释比
IHC-P3 - 5 µg/ml
WB0.3 - 1 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 9531 Human BAG3

Swiss-port # O95817 Human BAG family molecular chaperone regulator 3

背景介绍 BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
预测分子量 62 kDa

检测图片 (5) Click the Picture to Zoom In

  • ARG63391 anti-BAG3 antibody WB image

    Western blot: 30 µg of HeLa cell lysate stained with ARG63391 anti-BAG3 antibody at 1:1000 dilution.

  • ARG63391 anti-BAG3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63391 anti-BAG3 antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG63391 anti-BAG3 antibody WB image

    Western blot: 35 µg of MCF7 cell lysate (in RIPA buffer) stained with ARG63391 anti-BAG3 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.

  • ARG63391 anti-BAG3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63391 anti-BAG3 antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG63391 anti-BAG3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63391 anti-BAG3 antibody at 3.75 µg/ml dilution followed by AP-staining.

客户反馈

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anti-BAG3 antibody

Application:WB

Sample:293T

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC