ARG42744

anti-B7-H4 antibody

anti-B7-H4 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes B7-H4
反应物种 Hu
应用 FACS, ICC/IF, IHC-P
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 B7-H4
抗原物种 Human
抗原 Recombinant protein corresponding to T57-A258 of Human B7-H4.
偶联标记 Un-conjugated
別名 B7H4; B7 homolog 4; Protein B7S1; V-set domain-containing T-cell activation inhibitor 1; B7-H4; B7X; T-cell costimulatory molecule B7x; B7h.5; VCTN1; PRO1291; Immune costimulatory protein B7-H4; B7S1

应用说明

应用建议
应用 推荐稀释比
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
IHC-P1:200 - 1:1000
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
抗菌剂 0.05% Sodium azide
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 79679 Human VTCN1

Swiss-port # Q7Z7D3 Human V-set domain-containing T-cell activation inhibitor 1

基因名称 VTCN1
全名 V-set domain containing T cell activation inhibitor 1
背景介绍 This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
生物功能 Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. [UniProt]
细胞定位 Cell membrane; Single-pass type I membrane protein. Note=Expressed at the cell surface. A soluble form has also been detected. [UniProt]
预测分子量 31 kDa
翻译后修饰 N-glycosylated. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG42744 anti-B7-H4 antibody ICC/IF image

    Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG42744 anti-B7-H4 antibody (green) at 2 µg/ml dilutioin, overnight at 4°C. DAPI (blue) for nuclear staining.

  • ARG42744 anti-B7-H4 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42744 anti-B7-H4 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG42744 anti-B7-H4 antibody FACS image

    Flow Cytometry: U2OS cells were blocked with 10% normal goat serum and then stained with ARG42744 anti-B7-H4 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.