ARG55759
anti-B Raf antibody
anti-B Raf antibody for Flow cytometry,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes B Raf |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | B Raf |
| 抗原物种 | Human |
| 抗原 | Human B-RAF recombinant protein. |
| 偶联标记 | Un-conjugated |
| 別名 | BRAF1; v-Raf murine sarcoma viral oncogene homolog B1; Proto-oncogene B-Raf; p94; Serine/threonine-protein kinase B-raf; RAFB1; EC 2.7.11.1; B-RAF1; NS7 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P15056 Human Serine/threonine-protein kinase B-raf |
|---|---|
| 基因名称 | BRAF |
| 全名 | B-Raf proto-oncogene, serine/threonine kinase |
| 背景介绍 | This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. |
| 预测分子量 | 84 kDa |
| 翻译后修饰 | Phosphorylation at Ser-365 by SGK1 inhibits its activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF. |
检测图片 (2) Click the Picture to Zoom In
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ARG55759 anti-B Raf antibody WB image
Western blot: 35 µg of HeLa cell lysate stained with ARG55759 anti-B Raf antibody.
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ARG55759 anti-B Raf antibody FACS image
Flow Cytometry: CEM cells stained with ARG55759 anti-B Raf antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
