ARG54698

Name: anti-B Raf antibody
Brand: arigo Biolaboratories
SKU: ARG54698
Price: 399 USD
Availability: InStock
Rating: 3.0 (1 reviews)

anti-B Raf antibody

Datasheet

订购信息

包装

价格 (USD)

100 μl

399

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes B Raf
反应物种	Hu
预测物种	Ms, Chk
应用	FACS, ICC/IF, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	B Raf
抗原物种	Human
抗原	KLH-conjugated synthetic peptide corresponding to aa. 424-453 of Human B Raf (NP_004324.2).
偶联标记	Un-conjugated
別名	BRAF1; v-Raf murine sarcoma viral oncogene homolog B1; Proto-oncogene B-Raf; p94; Serine/threonine-protein kinase B-raf; RAFB1; EC 2.7.11.1; B-RAF1; NS7

应用说明

应用建议

应用	推荐稀释比
FACS	1:10 - 1:50
ICC/IF	1:10 - 1:50
IHC-P	Assay-dependent
WB	1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

纯化	Protein A purified
缓冲液	PBS and 0.09% (W/V) Sodium azide
抗菌剂	0.09% (W/V) Sodium azide
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 673 Human BRAF Swiss-port # P15056 Human Serine/threonine-protein kinase B-raf
基因名称	BRAF
全名	B-Raf proto-oncogene, serine/threonine kinase
背景介绍	This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
生物功能	Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. [From Uniprot]
细胞定位	Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
研究领域	Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
预测分子量	84 kDa
翻译后修饰	Phosphorylation at Ser-365 by SGK1 inhibits its activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.

检测图片 (4) Click the Picture to Zoom In

ARG54698 anti-B Raf antibody WB image

Western blot: 30 µg of U87 cell lysate stained with ARG54698 anti-B Raf antibody at 1:500 dilution.
ARG54698 anti-B Raf antibody ICC/IF image

Immunofluorescence: HeLa cells stained with ARG54698 anti-B Raf antibody. DAPI (blue) for nuclear staining.
ARG54698 anti-B Raf antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG54698 anti-B Raf antibody.
ARG54698 anti-B Raf antibody FACS image

Flow Cytometry: HeLa cells stained with ARG54698 anti-B Raf antibody (right histogram) or without primary antibody control (left histogram).