ARG58116
anti-Axin 2 antibody
anti-Axin 2 antibody for ICC/IF,Western blot and Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Axin 2 |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | ICC/IF, WB |
| 特异性 | This antibody does not cross-react with AXIN1. At least four isoforms of Axin2 are known to exist; this antibody will recognize all four. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Axin 2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around 20 aa (C-terminus) of Human Axin2. |
| 偶联标记 | Un-conjugated |
| 別名 | AXIL; Axil; Axin-2; Axin-like protein; Conductin; Axis inhibition protein 2; ODCRCS |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | WB: Mouse Lung tissue lysate | ||||||
| 实际分子量 | ~ 93 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AXIN2 |
| 全名 | axin 2 |
| 背景介绍 | The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008] |
| 生物功能 | Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B (By similarity). [UniProt] |
| 预测分子量 | 94 kDa |
| 翻译后修饰 | Probably phosphorylated by GSK3B and dephosphorylated by PP2A. ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway. Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG58116 anti-Axin 2 antibody ICC/IF image
Immunofluorescence: Rat lung cells stained with ARG58116 anti-Axin 2 antibody at 20 µg/ml dilution.
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ARG58116 anti-Axin 2 antibody WB image
Western blot: Mouse lung lysate stained with ARG58116 anti-Axin 2 antibody at 1 µg/ml dilution.
