ARG42651
anti-Arylsulfatase A antibody [4C10]
anti-Arylsulfatase A antibody [4C10] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Mouse Monoclonal antibody [4C10] recognizes Arylsulfatase A |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 4C10 |
| 同位型 | IgG2a |
| 靶点名称 | Arylsulfatase A |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 454-482 of Human Arylsulfatase A. (QALKQLQLLKAQLDAAVTFGPSQVARGED) |
| 偶联标记 | Un-conjugated |
| 別名 | ASA; Cerebroside-sulfatase; EC 3.1.6.8; Arylsulfatase A; MLD |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||
| 实际分子量 | ~ 60 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ARSA |
| 全名 | arylsulfatase A |
| 背景介绍 | The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2010] |
| 生物功能 | Hydrolyzes cerebroside sulfate. [UniProt] |
| 细胞定位 | Lysosome. [UniProt] |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD). [UniProt] |
检测图片 (5) Click the Picture to Zoom In
-
ARG42651 anti-Arylsulfatase A antibody [4C10] IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42651 anti-Arylsulfatase A antibody [4C10] at 1 µg/ml dilution, overnight at 4°C.
-
ARG42651 anti-Arylsulfatase A antibody [4C10] WB image
Western blot: 50 µg of samples under reducing conditions. A375, A549 and SMMC-7721 whole cell lysates stained with ARG42651 anti-Arylsulfatase A antibody [4C10] at 0.5 µg/ml dilution, overnight at 4°C.
-
ARG42651 anti-Arylsulfatase A antibody [4C10] FACS image
Flow Cytometry: Raji cells were blocked with 10% normal goat serum and then stained with ARG42651 anti-Arylsulfatase A antibody [4C10] (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Mouse IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
-
ARG42651 anti-Arylsulfatase A antibody [4C10] WB image
Western blot: 50 µg of samples under reducing conditions. Rat testis, Rat liver, Rat brain, Rat lung, Mouse testis, Mouse liver, Mouse brain, Mouse lung and HEPA1-6 whole cell lysates stained with ARG42651 anti-Arylsulfatase A antibody [4C10] at 0.5 µg/ml dilution, overnight at 4°C.
-
ARG42651 anti-Arylsulfatase A antibody [4C10] FACS image
Flow Cytometry: ANA-1 cells were blocked with 10% normal goat serum and then stained with ARG42651 anti-Arylsulfatase A antibody [4C10] (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Mouse IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
