ARG43203
anti-Argininosuccinate Lyase antibody
anti-Argininosuccinate Lyase antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Argininosuccinate Lyase |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Argininosuccinate Lyase |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-300 of Human Argininosuccinate Lyase (NP_000039.2). |
| 偶联标记 | Un-conjugated |
| 別名 | EC 4.3.2.1; Argininosuccinate lyase; ASAL; Arginosuccinase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HepG2, Mouse liver and Rat liver | ||||||||
| 实际分子量 | ~ 52 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide, 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ASL |
| 全名 | argininosuccinate lyase |
| 背景介绍 | This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | Acetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG43203 anti-Argininosuccinate Lyase antibody ICC/IF image
Immunofluorescence: NIH/3T3 cells stained with ARG43203 anti-Argininosuccinate Lyase antibody at 1:100 dilution.
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ARG43203 anti-Argininosuccinate Lyase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney tissue stained with ARG43203 anti-Argininosuccinate Lyase antibody at 1:100 dilution.
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ARG43203 anti-Argininosuccinate Lyase antibody WB image
Western blot: 25 µg of HepG2 cell lysate stained with ARG43203 anti-Argininosuccinate Lyase antibody at 1:1000 dilution.
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ARG43203 anti-Argininosuccinate Lyase antibody WB image
Western blot: 25 µg of Mouse liver lysates stained with ARG43203 anti-Argininosuccinate Lyase antibody at 1:1000 dilution.
