ARG10648

anti-Aquaporin 3 antibody

anti-Aquaporin 3 antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot,Flow cytometry and Human,Mouse,Rat

publication_link 参考文献3

概述

产品描述 Rabbit Polyclonal antibody recognizes Aquaporin 3
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, IHC-Fr, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Aquaporin 3
抗原物种 Human
抗原 Synthetic peptide around the C-terminus of Human Aquaporin 3. (EENVKLAHVKHKEQI)
偶联标记 Un-conjugated
別名 Aquaporin-3; GIL; Aquaglyceroporin-3; AQP-3

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF1 - 5 µg/ml
IHC-Fr1 - 5 µg/ml
IHC-P1 - 5 µg/ml
WB0.5 - 1 µg/ml
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS, 0.025% Sodium azide and 2.5% BSA.
抗菌剂 0.025% Sodium azide
稳定剂 2.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 11828 Mouse AQP3

GeneID: 360 Human AQP3

GeneID: 65133 Rat AQP3

基因名称 AQP3
全名 aquaporin 3 (Gill blood group)
背景介绍 This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011]
生物功能 Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism (By similarity). [UniProt]
预测分子量 32 kDa

检测图片 (7) Click the Picture to Zoom In

  • ARG10648 anti-Aquaporin 3 antibody ICC/IF image

    Immunocytochemistry: HeLa cells stained with ARG10648 anti-Aquaporin 3 antibody.

  • ARG10648 anti-Aquaporin 3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human renal cancer tissue. Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 20 min. The tissue section was stained with ARG10648 anti-Aquaporin 3 antibody.

  • ARG10648 anti-Aquaporin 3 antibody WB image

    Western blot: 1) Rat kidney, 2) Rat lung, 3) Mouse kidney, 4) MDA-MB-453, a human breast cancer line, and 5) SMMC-7221, a human hepatocarcinoma cell line. Lysates stained with ARG10648 anti-Aquaporin 3 antibody.

  • ARG10648 anti-Aquaporin 3 antibody FACS image

    Flow Cytometry: HeLa cells were blocked with goat sera and stained with ARG10648 anti-Aquaporin 3 antibody at 1 µg/10^6 cells (blue); Cells alone (red); Isotype control (green).

  • ARG10648 anti-Aquaporin 3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 20 min. The tissue section was stained with ARG10648 anti-Aquaporin 3 antibody.

  • ARG10648 anti-Aquaporin 3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat kidney tissue stained with ARG10648 anti-Aquaporin 3 antibody.

  • ARG10648 anti-Aquaporin 3 antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Rat kidney tissue stained with ARG10648 anti-Aquaporin 3 antibody.

文献引用

Pterostilbene Attenuates Particulate Matter-Induced Oxidative Stress, Inflammation and Aging in Keratinocytes

WB / Human

Wei-Lin Teng et al.
Antioxidants,  (2021)

publication_link

 

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Predicting the effects of radiotherapy based on diffusion kurtosis imaging in a xenograft mouse model of esophageal carcinoma.

WB / Human

An-Du Zhang et al.
Experimental and Therapeutic Medicine.,  (2021)

publication_link

 

hr_line

Improvement of Skin Penetration, Antipollutant Activity and Skin Hydration of 7,3',4'-Trihydroxyisoflavone Cyclodextrin Inclusion Complex.

WB / Human

Huang Pao-Hsien et al.
Pharmaceutics.,  (2019)

publication_link

 

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