ARG52229
anti-Aquaporin 2 phospho (Ser264) antibody
anti-Aquaporin 2 phospho (Ser264) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Rat
Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Aquaporin 2 phospho (Ser264) |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hu, Ms, Bov, Chk, Dog, NHuPrm |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Aquaporin 2 |
| 抗原物种 | Rat |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser264 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | Aquaporin-2; Aquaporin-CD; AQP-2; ADH water channel; Collecting duct water channel protein; Water channel protein for renal collecting duct; AQP-CD; WCH-CD |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | Specific for the ~29k AQP2 protein phosphorylated at Ser264 . Also recognizes the glycosylated form of AQP2 at ~ 37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AQP2 |
| 全名 | aquaporin 2 (collecting duct) |
| 背景介绍 | Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-264 has been identified as a novel phosphorylation site on AQP2 and shown to be regulated by vasopressin thus implicating this site as important in AQP2 trafficking and subcellular distribution (Fenton RA et al., 2008). |
| 研究领域 | Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 29 kDa |
| 翻译后修饰 | Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. |
检测图片 (1) Click the Picture to Zoom In
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ARG52229 anti-Aquaporin 2 phospho (Ser264) antibody WB image
Western blot: vasopressin (AVP) treated Rat kidney lysate showing specific immunolabeling of the ~29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser 264 stained with ARG52229 anti-Aquaporin 2 phospho (Ser264) antibody.
