ARG22360
anti-Aquaporin 2 antibody
anti-Aquaporin 2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
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概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Aquaporin 2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | Detects ~28.8 kDa. May detect larger glycosylated bands ~35-50 kDa. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Aquaporin 2 |
| 抗原物种 | Rat |
| 抗原 | Synthetic peptide around the C-terminus of Rat Aquaporin 2. (N-CLKGLEPDTDWEEREVRRRQ) |
| 偶联标记 | Un-conjugated |
| 別名 | Aquaporin-2; Aquaporin-CD; AQP-2; ADH water channel; Collecting duct water channel protein; Water channel protein for renal collecting duct; AQP-CD; WCH-CD |
应用说明
| 应用建议 |
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| 应用说明 | WB: 0.5 µg/ml of this antibody was sufficient for detection of aquaporin 2 in 10 µg of Rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:HRP as the secondary antibody. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS, 0.09% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Aqp2 |
| 全名 | aquaporin 2 |
| 背景介绍 | This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. [provided by RefSeq, Oct 2008] |
| 生物功能 | Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. [UniProt] |
| 细胞定位 | Apical cell membrane, Basolateral Cell Membrane, Cell membrane, Cytoplasmic Vesicle, Cytoplasmic vesicle membrane, Golgi apparatus |
| 预测分子量 | 28.8 kDa (unmodified); 35 - 50 kDa (glycosylated) |
| 翻译后修饰 | Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. |
检测图片 (2) Click the Picture to Zoom In
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ARG22360 anti-Aquaporin 2 antibody IHC image
Immunohistochemistry: Rat kidney tissue stained with ARG22360 anti-Aquaporin 2 antibody (green) at 1:200 dilution.
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ARG22360 anti-Aquaporin 2 antibody WB image
Western blot: Rat kidney inner medullary homogenates stained with ARG22360 anti-Aquaporin 2 antibody at 1:2000 dilution. Showing glycosylated and non-glycosylated bands.
客户反馈
Good
anti-Aquaporin 2 antibody
Application：WB
Sample：Mouse heart
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
