ARG40568
anti-Apolipoprotein E antibody
anti-Apolipoprotein E antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Apolipoprotein E |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Apolipoprotein E |
| 抗原物种 | Mouse |
| 抗原 | Recombinant protein corresponding to D55-Q294 of Mouse Apolipoprotein E. |
| 偶联标记 | Un-conjugated |
| 別名 | Apolipoprotein E; Apo-E; APO-E; LPG; AD2; LDLCQ5 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | APOE |
| 全名 | apolipoprotein E |
| 背景介绍 | The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
| 生物功能 | Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 预测分子量 | 36 kDa |
| 翻译后修饰 | Synthesized with the sialic acid attached by O-glycosidic linkage and is subsequently desialylated in plasma. O-glycosylated with core 1 or possibly core 8 glycans. Thr-307 and Ser-314 are minor glycosylation sites compared to Ser-308. Glycated in plasma VLDL of normal subjects, and of hyperglycemic diabetic patients at a higher level (2-3 fold). Phosphorylated by FAM20C in the extracellular medium. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG40568 anti-Apolipoprotein E antibody ICC/IF image
Immunofluorescence: HEPA 1-6 cells were blocked with 10% goat serum and then stained with ARG40568 anti-Apolipoprotein E antibody (green) at 5 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG40568 anti-Apolipoprotein E antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse liver tissue stained with ARG40568 anti-Apolipoprotein E antibody at 1 µg/ml dilution.
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ARG40568 anti-Apolipoprotein E antibody WB image
Western blot: Mouse spleen and Mouse kidney lysates stained with ARG40568 anti-Apolipoprotein E antibody at 0.5 µg/ml dilution.
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ARG40568 anti-Apolipoprotein E antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat liver tissue stained with ARG40568 anti-Apolipoprotein E antibody at 1 µg/ml dilution.
