ARG43616

anti-Annexin A1 antibody

anti-Annexin A1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Monkey

概述

产品描述 Rabbit Polyclonal antibody recognizes Annexin A1
反应物种 Hu, Mk
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Annexin A1
抗原物种 Human
抗原 Synthetic peptide corresponding to a.a. 3-24 of Human Annexin A1.
偶联标记 Un-conjugated
Protein Full name Annexin A1
別名 ANX1; Calpactin II; Phospholipase A2 inhibitory protein; Chromobindin-9; LPC1; Annexin-1; Annexin A1; Lipocortin I; Annexin I; p35; Calpactin-2

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 μg / 1X10^6 cells
ICC/IF1:50 - 1:100
IHC-P1:200 - 1:1000
WB1:500 - 1:4000
应用说明 IHC-P: Antigen Retrieval: By heat mediation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 37-39 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA.
抗菌剂 0.05% Thimerosal and 0.05% Sodium azide
稳定剂 5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 301 Human ANXA1

Swiss-port # P04083 Human Annexin A1

基因名称 ANXA1
全名 annexin A1
背景介绍 This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]
生物功能 Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. [UniProt]
细胞定位 Endosome; Nucleus; Plasma membrane; Secreted. [UniProt]
预测分子量 39 kDa
翻译后修饰 Phosphorylated by protein kinase C, EGFR and TRPM7 (PubMed:2457390, PubMed:15485879). Phosphorylated in response to EGF treatment (PubMed:2532504).

Sumoylated. [UniProt]

检测图片 (5) Click the Picture to Zoom In

  • ARG43616 anti-Annexin A1 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human placenta tissuer tissue stained withARG43616 anti-Annexin A1 antibody st 1:500 dulution.

  • ARG43616 anti-Annexin A1 antibody WB image

    Western blot: HeLa cell lysates stained withARG43616 anti-Annexin A1 antibody at 1:1000 dilution.

  • ARG43616 anti-Annexin A1 antibody ICC/IF image

    Immunofluorescence: A431 cells were stained with ARG43616 anti-Annexin A1 antibody at 1:100 dilution 30 minutes at 37°C.

  • ARG43616 anti-Annexin A1 antibody FACS image

    Flow Cytometry: A431 cells stained withARG43616 anti-Annexin A1 antibody at 1:100 dilution, 1 μg / 10^6 .

  • ARG43616 anti-Annexin A1 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil tissue stained withARG43616 anti-Annexin A1 antibody st 1:500 dulution.