ARG22241

anti-Ankyrin B antibody [S105-13]

anti-Ankyrin B antibody [S105-13] for ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat

publication_link 参考文献1

概述

产品描述 Mouse Monoclonal antibody [S105-13] recognizes Ankyrin B
反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC, WB
特异性 Detects ~200kDa. No cross reactivity against Ankyrin-G.
宿主 Mouse
克隆 Monoclonal
克隆号 S105-13
同位型 IgG1
靶点名称 Ankyrin B
抗原物种 Human
抗原 Synthetic peptide around aa. 203-496 of Human Ankyrin B
偶联标记 Un-conjugated
別名 Ankyrin-B; Non-erythroid ankyrin; ANK-2; Ankyrin-2; LQT4; brank-2; Brain ankyrin

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:200
IHC1:200
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
抗菌剂 0.09% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 109676 Mouse ANK2

GeneID: 287 Human ANK2

Swiss-port # Q01484 Human Ankyrin-2

Swiss-port # Q8C8R3 Mouse Ankyrin-2

基因名称 ANK2
全名 ankyrin 2, neuronal
背景介绍 This gene encodes a member of the ankyrin family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton. Ankyrins play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. The protein encoded by this gene is required for targeting and stability of Na/Ca exchanger 1 in cardiomyocytes. Mutations in this gene cause long QT syndrome 4 and cardiac arrhythmia syndrome. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Dec 2011]
生物功能 In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions (By similarity). Attaches integral membrane proteins to cytoskeletal elements. Also binds to cytoskeletal proteins. Required for coordinate assembly of Na/Ca exchanger, Na/K ATPase and InsP3 receptor at sarcoplasmic reticulum sites in cardiomyocytes. Required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) in the inner segment of rod photoreceptors. Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate. [UniProt]
细胞定位 Cytoplasm, Apical cell membrane, Cell membrane, cytoskeleton, M line, myofibril, Sarcomere
预测分子量 434 kDa
翻译后修饰 Phosphorylated at multiple sites by different protein kinases and each phosphorylation event regulates the protein's structure and function.

检测图片 (2) Click the Picture to Zoom In

  • ARG22241 anti-Ankyrin B antibody [S105-13] ICC/IF image

    Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ARG22241 anti-Ankyrin B antibody [S105-13] at 1:100 for 60 min at RT. Secondary Antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ARG22241 anti-Ankyrin B antibody [S105-13] (D) Composite.

  • ARG22241 anti-Ankyrin B antibody [S105-13] WB image

    Western blot: Rat brain membrane lysate stained with ARG22241 anti-Ankyrin B antibody [S105-13] at 1:1000 dilution.

文献引用

Downregulation of miR-146a Contributes to Cardiac Dysfunction Induced by the Tyrosine Kinase Inhibitor Sunitinib.

WB / Mouse

Shen Li et al.
Front. Pharmacol.,  (2019)

publication_link

 

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