ARG45225
anti-Angiomotin antibody
anti-Angiomotin antibody for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Angiomotin |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Angiomotin |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human AMOT. |
| 偶联标记 | Un-conjugated |
| 別名 | AMOT; Angiomotin; KIAA1071 |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 140 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AMOT |
| 全名 | Angiomotin |
| 背景介绍 | This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions. Repressor of YAP1 and WWTR1/TAZ transcription of target genes, potentially via regulation of Hippo signaling-mediated phosphorylation of YAP1 which results in its recruitment to tight junctions . [UniProt] |
| 细胞定位 | Cell junction; Tight junction. [UniProt] |
| 预测分子量 | 118 kDa |
| 翻译后修饰 | Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
-
ARG45225 Anti-Angiomotin antibody ICC/IF image
Immunofluorescence: CACO-2 stained with ARG45225 Anti-Angiomotin antibody at 5 ug/ml dilution.
-
ARG45225 Anti-Angiomotin antibody WB image
Western blot: 293T and Hela stained with ARG45225 Anti-Angiomotin antibody at 0.5 μg/ml dilution.
-
ARG45225 Anti-Angiomotin antibody FACS image
Flow Cytometry: CACO-2 stained with ARG45225 Anti-Angiomotin antibody at 1 µg/10^6 cells dilution.
