ARG43659
anti-AlaRS / Alanyl tRNA Synthetase antibody
anti-AlaRS / Alanyl tRNA Synthetase antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes AlaRS / Alanyl tRNA Synthetase |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | AlaRS / Alanyl tRNA Synthetase |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within a.a. 1-150 of Human Alanyl-tRNA Synthetase. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Alanine--tRNA ligase, cytoplasmic |
| 別名 | Renal carcinoma antigen NY-REN-42; CMT2N; EIEE29; AlaRS; EC 6.1.1.7; Alanine--tRNA ligase, cytoplasmic; Alanyl-tRNA synthetase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | WB: HeLa cell lysates; IHC: Human lung cancer tissue. | ||||||||
| 实际分子量 | 110-120 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS, 0.01% Thiomersal and 50% Glycerol. |
| 抗菌剂 | 0.01% Thiomersal |
| 稳定剂 | 50% Glycerol |
| 浓度 | Batch dependent |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AARS |
| 全名 | alanyl-tRNA synthetase |
| 背景介绍 | The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq, Jul 2008] |
| 生物功能 | Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. [UniProt] |
| 细胞定位 | Cytoplasm [UniProt] |
| 预测分子量 | 107 kDa |
| 翻译后修饰 | ISGylated. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human Lung tissue stained with ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody at 1:100 dilution.
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ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody ICC/IF image
Immunofluorescence: 293T cells were stained with ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody at 1:100.
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ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody IHC-P image WB image
Western blot: HeLa cell lysates stained with ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody at 1:3000 dilution
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ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat Spleen tissue stained with ARG43659 anti-AlaRS / Alanyl tRNA Synthetase antibody at 1:100 dilution.
