ARG40253

anti-Adipophilin antibody

anti-Adipophilin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes Adipophilin
反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Adipophilin
抗原物种 Human
抗原 Recombinant fusion protein corresponding to aa. 168-437 of Human Adipophilin (NP_001113.2).
偶联标记 Un-conjugated
別名 ADRP; Adipophilin; Adipose differentiation-related protein; ADFP; Perilipin-2

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:100 - 1:500
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 SW620, Mouse heart and Rat heart
实际分子量 ~ 50 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 11520 Mouse PLIN2

GeneID: 123 Human PLIN2

Swiss-port # P43883 Mouse Perilipin-2

Swiss-port # Q99541 Human Perilipin-2

基因名称 PLIN2
全名 perilipin 2
背景介绍 The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]
生物功能 May be involved in development and maintenance of adipose tissue. [UniProt]
细胞定位 Membrane; Peripheral membrane protein. Lipid droplet. [UniProt]
预测分子量 48 kDa
翻译后修饰 Acylated; primarily with C14, C16 and C18 fatty acids. [UniProt]

检测图片 (4) Click the Picture to Zoom In

  • ARG40253 anti-Adipophilin antibody IHC-P image

    Immunohistochemistry: Human liver stained with ARG40253 anti-Adipophilin antibody at 1:500 dilution.

  • ARG40253 anti-Adipophilin antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG40253 anti-Adipophilin antibody at 1:100 dilution.

  • ARG40253 anti-Adipophilin antibody WB image

    Western blot: 25 µg of SW620 cell lysate stained with ARG40253 anti-Adipophilin antibody at 1:1000 dilution.

  • ARG40253 anti-Adipophilin antibody WB image

    Western blot: 25 µg of Rat heart and Mouse heart lysates stained with ARG40253 anti-Adipophilin antibody at 1:1000 dilution.