ARG22620
anti-Acrolein antibody [10A10]
anti-Acrolein antibody [10A10] for Flow cytometry,ICC/IF,Western blot and Other
概述
| 产品描述 | Mouse Monoclonal antibody [10A10] recognizes Acrolein |
|---|---|
| 反应物种 | Other |
| 应用 | FACS, ICC/IF, WB |
| 特异性 | Specific for Acrolein modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal or Malondialdehyde. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 10A10 |
| 同位型 | IgG1 |
| 靶点名称 | Acrolein |
| 抗原物种 | Others |
| 抗原 | Synthetic Acrolein modified KLH. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.9% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.9% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
检测图片 (4) Click the Picture to Zoom In
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ARG22620 anti-Acrolein antibody [10A10] ICC/IF image
Immunofluorescence: 5% Formaldehyde fixed (5 min) HEK293 cells stained with ARG22620 anti-Acrolein antibody [10A10] at 1:50 for 30 - 60 min at RT. Secondary Antibody: Goat anti-Mouse (Alexa Fluor 488) at 1:1500 for 30 - 60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain. (A,C,E,G) Untreated. (B,D,F,H) Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Primary antibody. (G,H) Composite.
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ARG22620 anti-Acrolein antibody [10A10] FACS image
Flow Cytometry: 90% Methanol fixed Neuroblastoma cells (SH-SY5Y) stained with ARG22620 anti-Acrolein antibody [10A10] at 1:50 for 30 min on ice. Secondary Antibody: Goat anti-Mouse (PE) at 1:100 for 20 min at RT. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours. Dashed line: Non specific IgG control. Solid line: Primary antibodies.
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ARG22620 anti-Acrolein antibody [10A10] WB image
Western blot: Acrolein-BSA (0.5 µg), Acrolein-BSA (2.0 µg), BSA (0.5 µg), and BSA (2.0 µg) stained with ARG22620 anti-Acrolein antibody [10A10] at 1:1000 for 2 hours at RT.
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ARG22620 anti-Acrolein antibody [10A10] WB image
Western blot: 12 µg of HeLa cell lysate and H2O2 treated HeLa cell lysate stained with ARG22620 anti-Acrolein antibody [10A10] at 1:1000 for 2 hours at RT.
