ARG20523

anti-Acetylated Lysine antibody

Datasheet

参考文献2

订购信息

包装

价格 (USD)

50 μg

335

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes Acetylated Lysine
反应物种	Other
应用	ELISA, ICC/IF, IP, WB
特异性	Detects proteins containing acetylated lysine residues. Does not detect non-acetylated lysine residues.
宿主	Rabbit
克隆	Polyclonal
靶点名称	Acetylated Lysine
抗原	Acetylated KLH
偶联标记	Un-conjugated

应用说明

交叉反应性说明

Species Independent.

应用建议

应用	推荐稀释比
ELISA	Assay-dependent
ICC/IF	1:100
IP	Assay-dependent
WB	1:250

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS, 0.09% Sodium azide and 50% Glycerol
抗菌剂	0.09% Sodium azide
稳定剂	50% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

背景介绍	Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5). 1. Yang XJ. (2005). Oncogene. 24:1653-1662. 2. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308. 3. Yang XJ. (2004). Bioessays 26:1076-1087. 4. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143. 5. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218. 6. Chan, H.M. et al. (2001). Nat. Cell Biol. 3: 667- . 674. 7. Martinez-Balbas, M.A. et al. (2000). EMBO J. 19: 662-671.
研究领域	Gene Regulation antibody

背景介绍

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).

1. Yang XJ. (2005). Oncogene. 24:1653-1662.
2. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308.
3. Yang XJ. (2004). Bioessays 26:1076-1087.
4. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143.
5. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218.
6. Chan, H.M. et al. (2001). Nat. Cell Biol. 3: 667- . 674.
7. Martinez-Balbas, M.A. et al. (2000). EMBO J. 19: 662-671.

研究领域

Gene Regulation antibody

检测图片 (4) Click the Picture to Zoom In

ARG20523 anti-Acetylated Lysine antibody ICC/IF image

Immunofluorescence: Heat Shocked (42°C for 1 hour) HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ARG20523 anti-Acetylated Lysine antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain. Middle: ARG20523 anti-Acetylated Lysine antibody. Right: Composite.
ARG20523 anti-Acetylated Lysine antibody WB image

Western blot: acetylated histone from TSA-treated mouse spleen cells, stained with ARG20523 anti-Acetylated Lysine antibody.
ARG20523 anti-Acetylated Lysine antibody ICC/IF image

Immunofluorescence: Heat Shocked (42°C for 1 hour) HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ARG20523 anti-Acetylated Lysine antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain. Middle: ARG20523 anti-Acetylated Lysine antibody. Right: Composite.
ARG20523 anti-Acetylated Lysine antibody WB image

Western blot: acetylated lysine in BSA (Left) and Acetylated BSA (Right) stained with ARG20523 anti-Acetylated Lysine antibody at 1:1000 dilution.

文献引用

Sirtuin 6 Deacetylates Apoptosis-Associated Speck-Like Protein (ASC) to Inhibit Endothelial Cell Pyroptosis in Atherosclerosis

WB / Human

Jian Huang et al.
Int Heart J., (2024)

Interfering TRIB3 protects the blood brain barrier through PI3K/Akt pathway to alleviate cerebral ischemia-reperfusion injury in diabetes mellitus mice

IP / Mouse

Heng-Jun Zhou et al.
Chem Biol Interact., (2024)