ARG20521

anti-Acetylated Lysine antibody [7F8]

Datasheet

订购信息

包装

价格 (USD)

50 μg

335

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

产品说明书

概述

产品描述	Mouse Monoclonal antibody [7F8] recognizes Acetylated Lysine
反应物种	Other
应用	ELISA, ICC/IF, IHC-P, IP, WB
特异性	Detects proteins containing acetylated lysine residues. Does not detect non-acetylated lysine residues.
宿主	Mouse
克隆	Monoclonal
克隆号	7F8
同位型	IgG1
靶点名称	Acetylated Lysine
抗原	Acetylated KLH
偶联标记	Un-conjugated

应用说明

交叉反应性说明

Species Independent. Bovine albumin and Avian histones have been tested.

应用建议

应用	推荐稀释比
ELISA	Assay-dependent
ICC/IF	Assay-dependent
IHC-P	Assay-dependent
IP	Assay-dependent
WB	1:200 - 1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
抗菌剂	0.09% Sodium azide
稳定剂	50% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

背景介绍	Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine N ε - acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anticancer drugs currently in development (5). 1. Yang XJ. (2005). Oncogene. 24:1653-1662. 2. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308. 3. Yang XJ. (2004). Bioessays 26:1076-1087. 4. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143. 5. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218.
研究领域	Gene Regulation antibody

背景介绍

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine N ε - acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anticancer drugs currently in development (5).

1. Yang XJ. (2005). Oncogene. 24:1653-1662.
2. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308.
3. Yang XJ. (2004). Bioessays 26:1076-1087.
4. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143.
5. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218.

研究领域

Gene Regulation antibody

检测图片 (3) Click the Picture to Zoom In

ARG20521 anti-Acetylated Lysine antibody [7F8] IHC-P image

Immunohistochemistry: Bouin's fixed and paraffin-embedded Mouse backskin stained with ARG20521 anti-Acetylated Lysine antibody [7F8] at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat anti-Mouse (green) at 1:50 for 1 hour at RT.
ARG20521 anti-Acetylated Lysine antibody [7F8] WB image

Western blot: A549 cell lysate stained with ARG20521 anti-Acetylated Lysine antibody [7F8] at 1:200 dilution.
ARG20521 anti-Acetylated Lysine antibody [7F8] WB image

Western blot: Acetylated lysine in 1) 75 ng of Acetylated BSA, 2) Non-acetylated BSA, and 3) Marker. The blots were stained with ARG20521 anti-Acetylated Lysine antibody [7F8] at 1:1000 dilution.