ARG52233
anti-ATRIP phospho (Ser239) antibody
anti-ATRIP phospho (Ser239) antibody for Western blot and Mouse
Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATRIP phospho (Ser239) |
|---|---|
| 反应物种 | Ms |
| 预测物种 | Hu, Bov, NHuPrm, Sheep |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATRIP |
| 抗原物种 | Human |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser239 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | ATR-interacting protein; ATM and Rad3-related-interacting protein |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | Specific for the ~86k ATRIP protein phosphorylated at Ser239. The antibody also recognizes a band at ~32k. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ATRIP |
| 全名 | ATR interacting protein |
| 背景介绍 | ATRIP, ATR interacting protein, binds to ATR to regulate ATR expression, and is an essential component of the DNA damage checkpoint pathway (Cortez et al, 2001). ATR is recruited to DNA lesions in part through its association with ATRIP, which in turn interacts with the single-stranded DNA binding protein RPA (Ball et al, 2007). DNA replication forks may stall as a result of DNA damage causing phosphorylation of several proteins, including BRCA1 when colocalizing with ATR/ATRIP complex and RPA (Venere et al, 2007). The DNA replication fork stall coincides with BRCA1 directly phosphorylating ATRIP at ser239 (Venere et al, 2007). |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 86 kDa |
| 翻译后修饰 | Phosphorylated by ATR. |
检测图片 (1) Click the Picture to Zoom In
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ARG52233 anti-ATRIP phospho (Ser239) antibody WB image
Western blot: Mouse testis lysate showing specific immunolabeling of the ~86kd ATRIP protein phosphorylated at Ser 239 (control) stained with ARG52233 anti-ATRIP phospho (Ser239) antibody.
Phosphospecificity is shown in the second lane (lambda-phosphatase: λ-Ptase).
The blot is identical to the control except that the lysate was incubated in λ-Ptase (800 units/1mg protein for 30 min).
The immunolabeling is completely eliminated by treatment with λ-Ptase.
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