ARG54457
anti-ATR antibody
anti-ATR antibody for Western blot and Human,Mouse
Cancer antibody; Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATR |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | WB |
| 特异性 | This antibody reacts with human and mouse. Antibody is affinity purified. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATR |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide representing a portion of the protein encoded within exon 5 (LocusLink ID 545). |
| 偶联标记 | Un-conjugated |
| 別名 | FRP1; SCKL; FCTCS; FRAP-related protein 1; Serine/threonine-protein kinase ATR; EC 2.7.11.1; MEC1; SCKL1; Ataxia telangiectasia and Rad3-related protein |
应用说明
| 应用说明 | Western blot: 0.033 - 0.067 ug/ml. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | Tris-citrate/phosphate (pH 7-8) and 0.1% Sodium azide |
| 抗菌剂 | 0.1% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q13535 Human Serine/threonine-protein kinase ATR |
|---|---|
| 基因名称 | ATR |
| 全名 | ATR serine/threonine kinase |
| 背景介绍 | The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq, Jul 2008] |
| 生物功能 | Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. [UniProt] |
| 研究领域 | Cancer antibody; Gene Regulation antibody |
| 预测分子量 | 301 kDa |
| 翻译后修饰 | Phosphorylated; autophosphorylates in vitro. |
