ARG55614
anti-ATP5H antibody
anti-ATP5H antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATP5H |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Bov |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATP5H |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 68-97 (Center) of Human ATP5H. |
| 偶联标记 | Un-conjugated |
| 別名 | ATPQ; ATP synthase subunit d, mitochondrial; ATPase subunit d |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HepG2 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O75947 Human ATP synthase subunit d, mitochondrial |
|---|---|
| 基因名称 | ATP5H |
| 全名 | ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d |
| 背景介绍 | Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010] |
| 生物功能 | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. [UniProt] |
| 细胞定位 | Mitochondrion. Mitochondrion inner membrane. |
| 研究领域 | Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 18 kDa |
检测图片 (3) Click the Picture to Zoom In
-
ARG55614 anti-ATP5H antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain tissue stained with ARG55614 anti-ATP5H antibody.
-
ARG55614 anti-ATP5H antibody WB image
Western blot: 35 µg of HepG2 cell lysate stained with ARG55614 anti-ATP5H antibody.
-
ARG55614 anti-ATP5H antibody FACS image
Flow Cytometry: HepG2 cells stained with ARG55614 anti-ATP5H antibody (right histogram) or without primary antibody as control (left histogram), followed by incubation with FITC labelled secondary antibody.
